Magnetic sensors for protease assays

被引：192

作者：

Zhao, M ^{[1
]}

Josephson, L ^{[1
]}

Tang, Y ^{[1
]}

Weissleder, R ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Mol Imaging Res, Charlestown, MA 02129 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 12期

关键词：

biosensors; biotin; enzymes; magnetic properties; proteases;

D O I：

10.1002/anie.200390352

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Superparamagnetic nanosensors conjugated to avidin (CLIO-A) can be used to measure sensitively and quantitively the proteolytic cleavage of bi-biotinylated peptide substrates (rods in the scheme) by changes in magnetic resonance properties. This approach may be generalized to determine the activity of a wide range of hydrolytic enzymes.

引用

页码：1375 / 1378

页数：4

共 24 条

[1] Oligomerization of Paramagnetic Substrates Result in Signal Amplification and can be Used for MR Imaging of Molecular Targets [J].

Bogdanov, Alexei, Jr. ;

Matuszewski, Lars ;

Bremer, Christoph ;

Petrovsky, Alexander ;

Weissleder, Ralph .

MOLECULAR IMAGING, 2002, 1 (01) :16-23

[2]

Cha HJ, 1996, CANCER RES, V56, P2281

[3] A unique substrate recognition profile for matrix metalloproteinase-2 [J].

Chen, EI ;

Kridel, SJ ;

Howard, EW ;

Li, WZ ;

Godzik, A ;

Smith, JW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (06) :4485-4491

[4] Matrix metalloproteinases in vascular remodeling and atherogenesis - The good, the bad, and the ugly [J].

Galis, ZS ;

Khatri, JJ .

CIRCULATION RESEARCH, 2002, 90 (03) :251-262

[5]

Giannelli G, 2002, HISTOL HISTOPATHOL, V17, P339, DOI 10.14670/HH-17.339

[6] Design and synthesis of fluorogenic trypsin peptide substrates based on resonance energy transfer [J].

Grahn, S ;

Ullmann, D ;

Jakubke, HD .

ANALYTICAL BIOCHEMISTRY, 1998, 265 (02) :225-231

[7] High throughput magnetic resonance imaging for evaluating targeted nanoparticle probes [J].

Högemann, D ;

Ntziachristos, V ;

Josephson, L ;

Weissleder, R .

BIOCONJUGATE CHEMISTRY, 2002, 13 (01) :116-121

[8] A CONTINUOUS FLUORESCENCE-BASED ASSAY OF HUMAN CYTOMEGALOVIRUS PROTEASE USING A PEPTIDE SUBSTRATE [J].

HOLSKIN, BP ;

BUKHTIYAROVA, M ;

DUNN, BM ;

BAUR, P ;

DECHASTONAY, J ;

PENNINGTON, MW .

ANALYTICAL BIOCHEMISTRY, 1995, 227 (01) :148-155

[9]

IRVINE GB, 1990, ANAL BIOCHEM, V85, P304

[10] Calprotectin inhibits matrix metalloproteinases by sequestration of zinc [J].

Isaksen, B ;

Fagerhol, MK .

JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY, 2001, 54 (05) :289-292

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