Functional analysis of the catalytic subunit of Dictyostelium PKA in vivo

被引:9
作者
Dammann, H
Traincard, F
Anjard, C
van Bemmelen, MXP
Reymond, C
Véron, M
机构
[1] Inst Pasteur, Unite Regulat Enzymat Activ Cellulaires, F-75724 Paris 15, France
[2] Univ Lausanne, Inst Biol Cellulaire & Morphol, CH-1005 Lausanne, Switzerland
基金
澳大利亚研究理事会;
关键词
cAMP-dependent protein kinase (PKA; cAPK); Dictyostelium discoideum; development;
D O I
10.1016/S0925-4773(98)00025-2
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The catalytic subunit of the CAMP-dependent protein kinase (PKA) from Dictyostelium discoideum contains several domains, including an unusually long N-terminal extension preceding a highly conserved catalytic core. We transformed the aggregationless PkaC-null strain with several deletion constructs of both domains. Strains transformed with genes expressing catalytically-inactive polypeptides could not rescue development. Cotransformation with constructs encoding the N-terminal extension and the catalytic core, both unable to rescue development by themselves, yielded transformants able to proceed to late development. A 27-amino acid long hydrophobic region, immediately upstream of the catalytic core, was found indispensable for PKA function. A putative role of this sequence in the acquisition of the active conformation of the protein is discussed. (C) 1998 Elsevier Science Ireland Ltd.
引用
收藏
页码:149 / 157
页数:9
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