Isolation of high-purity casticin from Artemisia annua L. by high-speed counter-current chromatography

被引：47

作者：

Han, Xiao ^{[1
]}

Ma, Xiaofeng

Zhang, Tianyou

Zhang, Yabin

Liu, Qinghui

Ito, Yoichiro

机构：

[1] Beijing UE Biotech Co Ltd, Chinese Key Lab Natl Reference Mat Nat Prod, Beijing 100035, Peoples R China

[2] Grad Univ Chinese Acad Sci, Dept Biol, Beijing 100049, Peoples R China

[3] NHLBI, Ctr Biochem & Biophys, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1151卷 / 1-2期

关键词：

counter-current chromatography; preparative chromatography; Artemisia annua L; casticin;

D O I：

10.1016/j.chroma.2007.02.105

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Following an initial clean-up step on silica, high-speed counter-current chromatography (HSCCC) was used to purify a flavone, casticin (5,3 '-dihydroxy-3,6,7, 4 '-tetramethoxyflavone), from an extract of the dried leaves of Artemisia annua L. The two-phase solvent system used was composed of n-hexane-ethyl acetate-methanol-water at an optimized volume ratio of 7:10:7:10 (v/v). HSCCC separation of 226.4 mg of crude sample (containing casticin at 16.5% purity after silica gel clean-up) yielded 36.3 mg of casticin with a purity of over 99% and 96.2% recovery. Identification of the target compound was performed by H-1 NMR, C-13 NMR, two-dimensional NMR, electrospray ionization MS, IR and UV. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：180 / 182

页数：3

共 5 条

[1]

The flavonoid Casticin has multiple mechanisms of tumor cytotoxicity action [J].