Novel, highly expressed late nodulin gene (LjNOD16) from Lotus japonicus

被引:22
作者
Kapranov, P
deBruijn, FJ
Szczyglowski, K
机构
[1] MICHIGAN STATE UNIV,DEPT ENERGY,PLANT RES LAB,E LANSING,MI 48824
[2] MICHIGAN STATE UNIV,GENET PROGRAM,E LANSING,MI 48824
[3] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824
关键词
D O I
10.1104/pp.113.4.1081
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have isolated a Lotus japonicus cDNA corresponding to a highly abundant, late nodule-specific RNA species that encodes a polypeptide with a predicted molecular mass of 15.6 kD. The protein and its corresponding gene were designated Nlj16 and LjNOD16, respectively. LjNOD16 was found to be expressed only in the infected cells of L. japonicus nodules. Related DNA sequences could be identified in the genomes of both Glycine max and Medicago sativa. In the latter, a homologous mRNA species was detected in the nodules. Unlike LjNOD16, its alfalfa homologs appear to represent low-abundance mRNA species. However, the proteins corresponding to the LjNOD16 and its alfalfa homolog could be detected at similar levels in nodules but not in roots of both legume species. The predicted amino acid sequence analysis of nodulin Nlj16 revealed the presence of a long alpha-helical region and a positively charged C terminus. The former domain has a very high propensity to form a coiled-coil type structure, indicating that nodulin Nlj16 may interact with an as-yet-unidentified protein target(s) in the nodule-infected cells. Homology searches revealed no significant similarities to any known sequences in the databases, with the exception of two related, anonymous Arabidopsis expressed sequence tags.
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页码:1081 / 1090
页数:10
相关论文
共 53 条
[1]  
Almeida Engler J. de, 1994, Plant Molecular Biology Reporter, V12, P321, DOI 10.1007/BF02669275
[2]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[3]   CDNA SEQUENCE AND DIFFERENTIAL EXPRESSION OF THE GENE ENCODING THE GLUTAMINE-SYNTHETASE GAMMA-POLYPEPTIDE OF PHASEOLUS-VULGARIS L [J].
BENNETT, MJ ;
LIGHTFOOT, DA ;
CULLIMORE, JV .
PLANT MOLECULAR BIOLOGY, 1989, 12 (05) :553-565
[4]   NODULIN-35 - A SUBUNIT OF SPECIFIC URICASE (URICASE-II) INDUCED AND LOCALIZED IN THE UNINFECTED CELLS OF SOYBEAN NODULES [J].
BERGMANN, H ;
PREDDIE, E ;
VERMA, DPS .
EMBO JOURNAL, 1983, 2 (12) :2333-2339
[5]  
BORON L, 1989, ACTA BIOCHIM POL, V36, P295
[6]   CLONING AND CHARACTERIZATION OF A NODULE-ENHANCED GLUTAMINE SYNTHETASE-ENCODING GENE FROM LUPINUS-LUTEUS [J].
BORON, LJ ;
LEGOCKI, AB .
GENE, 1993, 136 (1-2) :95-102
[7]   ORGANIZATION AND EXPRESSION OF LEGHEMOGLOBIN GENES [J].
BRISSON, N ;
POMBOGENTILE, A ;
VERMA, DPS .
CANADIAN JOURNAL OF BIOCHEMISTRY, 1982, 60 (03) :272-278
[8]   CONTROL OF LEGHAEMOGLOBIN SYNTHESIS IN SNAKE BEANS [J].
BROUGHTON, WJ ;
DILWORTH, MJ .
BIOCHEMICAL JOURNAL, 1971, 125 (04) :1075-+
[9]  
Cas R.A. F., 1987, Volcanic Successions Modern and Ancient, DOI [10.1007/978-94-009-0951-9_6, DOI 10.1007/978-94-009-0951-9_6]
[10]   GENOMIC SEQUENCING [J].
CHURCH, GM ;
GILBERT, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995