Antiinflammatory Effect of Phytosterols in Experimental Murine Colitis Model: Prevention, Induction, Remission Study

被引：91

作者：

Aldini, Rita ^{[1
]}

Micucci, Matteo ^{[1
]}

Cevenini, Monica ^{[2
]}

Fato, Romana ^{[1
]}

Bergamini, Christian ^{[1
]}

Nanni, Cristina ^{[3
]}

Cont, Massimiliano ^{[3
]}

Camborata, Cecilia ^{[4
]}

Spinozzi, Silvia ^{[4
]}

Montagnani, Marco ^{[2
]}

Roda, Giulia ^{[2
]}

D'Errico-Grigioni, Antonia ^{[5
]}

Rosini, Francesca ^{[5
]}

Roda, Aldo ^{[4
]}

Mazzella, Giuseppe ^{[2
]}

Chiarini, Alberto ^{[1
]}

Budriesi, Roberta ^{[1
]}

机构：

[1] Univ Bologna, Dept Pharm & Biotecnol, Bologna, Italy

[2] Univ Bologna, Dept Med & Surg, Policlin S Orsola, Bologna, Italy

[3] Univ Bologna, Dept Nucl Med, Azienda Osped, Policlin S Orsola Malpighi, Bologna, Italy

[4] Univ Bologna, Dept Chem Giacomo Ciamician, Bologna, Italy

[5] Univ Bologna, DIMES Dept, Policlin S Orsola, Bologna, Italy

来源：

PLOS ONE | 2014年 / 9卷 / 09期

关键词：

NF-KAPPA-B; INTESTINAL EPITHELIAL-CELLS; SULFATE-INDUCED COLITIS; BETA-SITOSTEROL; PLANT STEROLS; IN-VITRO; CHOLESTEROL-METABOLISM; PENTACYCLIC TRITERPENE; MUSCARINIC RECEPTORS; PROTIUM-HEPTAPHYLLUM;

D O I：

10.1371/journal.pone.0108112

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Phytosterols, besides hypocholesterolemic effect, present anti-inflammatory properties. Little information is available about their efficacy in Inflammatory Bowel Disease (IBD). Therefore, we have evaluated the effect of a mixture of phytosterols on prevention/induction/remission in a murine experimental model of colitis. Phytosterols were administered x os before, during and after colitis induction with Dextran Sodium Sulfate (DSS) in mice. Disease Activity Index (DAI), colon length, histopathology score, F-18-FDG microPET, oxidative stress in the intestinal tissue (ileum and colon) and gallbladder ileum and colon spontaneous and carbachol (CCh) induced motility, plasma lipids and plasma, liver and biliary bile acids (BA) were evaluated. A similar longitudinal study was performed in a DSS colitis control group. Mice treated with DSS developed severe colitis as shown by DAI, colon length, histopathology score, F-18-FDG microPET, oxidative stress. Both spontaneous and induced ileal and colonic motility were severely disturbed. The same was observed with gallbladder. DSS colitis resulted in an increase in plasma cholesterol, and a modification of the BA pattern. Phytosterols feeding did not prevent colitis onset but significantly reduced the severity of the disease and improved clinical and histological remission. It had strong antioxidant effects, almost restored colon, ileal and gallbladder motility. Plasmatic levels of cholesterol were also reduced. DSS induced a modification in the BA pattern consistent with an increase in the intestinal BA deconjugating bacteria, prevented by phytosterols. Phytosterols seem a potential nutraceutical tool for gastrointestinal inflammatory diseases, combining metabolic systematic and local anti-inflammatory effects.

引用

页数：16

共 75 条

[1]

Curcuma longa Extract Exerts a Myorelaxant Effect on the Ileum and Colon in a Mouse Experimental Colitis Model, Independent of the Anti-Inflammatory Effect [J].