An assay for ascorbic acid based on polyaniline-coated microplates

被引：113

作者：

Bossi, A ^{[1
]}

Piletsky, SA

Piletska, EV

Righetti, PG

Turner, APF

机构：

[1] Cranfield Inst Technol, Inst Biosci & Technol, Cranfield MK43 0AL, Beds, England

[2] Univ Verona, Dipartimento Sci & Tecnol, I-37134 Verona, Italy

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 18期

关键词：

D O I：

10.1021/ac000185s

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A technique for modification of the microtiter reader plates well with a polyaniline (PANI) film sensitive for ascorbic acid is presented. The principle of the analyte detection is based on monitoring the changes in optical absorption of the PANI film resulting from the reduction process initiated by ascorbic acid. The detection limit for ascorbic acid is 1 mg/L. Testing with real samples (soft drinks, fruit juices) gave good correlation of the method with iodimetric titration. High sensitivity, stability, and good reproducibility of the measurements make the proposed system an attractive alternative to traditional assays, used in medicine, ecology and biotechnology.

引用

页码：4296 / 4300

页数：5

共 27 条

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