Electrochemical Detection in a Paper-Based Separation Device

被引:181
作者
Carvalhal, Rafaela Fernanda [1 ,2 ]
Kfouri, Marta Simao [3 ]
de Oliveira Piazetta, Maria Helena [3 ]
Gobbi, Angelo Luiz [3 ]
Kubota, Lauro Tatsuo [1 ,2 ]
机构
[1] State Univ Campinas UNICAMP, Inst Chem, Dept Analyt Chem, Campinas, SP, Brazil
[2] State Univ Campinas UNICAMP, Inst Chem, Inst Nacl Ciencia & Tecnol Bioanalit, Campinas, SP, Brazil
[3] LNLS, Microfabricat Lab, Campinas, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
MICROFLUIDIC DEVICES; LOW-COST; WAX;
D O I
10.1021/ac902647r
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Prototypes of microfluidic paper-based separation devices with amperometric detection were developed and evaluated. Photolithography was used to make a gold electrochemical microcell on polyester and that microcell was coupled to a strip of paper where a chromatographic separation occurs. The device performance was demonstrated with the separation and quantification of uric and ascorbic acid in mixtures. The method provides an analytical alternative for the determination of compounds where low cost and simplicity are essential.
引用
收藏
页码:1162 / 1165
页数:4
相关论文
共 22 条
[1]   Screening method for inherited disorders of purine and pyrimidine metabolism by capillary electrophoresis with reversed electroosmotic flow [J].
Adam, T ;
Lochman, P ;
Friedecky, D .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 767 (02) :333-340
[2]  
[Anonymous], 1987, ANALYST, V112, P199
[3]  
BOYLE R, 1972, INDICATOTS
[4]   Low-cost printing of poly(dimethylsiloxane) barriers to define microchannels in paper [J].
Bruzewicz, Derek A. ;
Reches, Meital ;
Whitesides, George M. .
ANALYTICAL CHEMISTRY, 2008, 80 (09) :3387-3392
[5]   Paper Microzone Plates [J].
Carrilho, Emanuel ;
Phillips, Scott T. ;
Vella, Sarah J. ;
Martinez, Andres W. ;
Whitesides, George M. .
ANALYTICAL CHEMISTRY, 2009, 81 (15) :5990-5998
[6]   Understanding Wax Printing: A Simple Micropatterning Process for Paper-Based Microfluidics [J].
Carrilho, Emanuel ;
Martinez, Andres W. ;
Whitesides, George M. .
ANALYTICAL CHEMISTRY, 2009, 81 (16) :7091-7095
[7]   PAPER CHROMATOGRAPHY [J].
CLEGG, DL .
ANALYTICAL CHEMISTRY, 1950, 22 (01) :48-59
[8]   Electrochemical Detection for Paper-Based Microfluidics [J].
Dungchai, Wijitar ;
Chailapakul, Orawon ;
Henry, Charles S. .
ANALYTICAL CHEMISTRY, 2009, 81 (14) :5821-5826
[9]   Fast HPLC-ECD analysis of ascorbic acid, dehydroascorbic acid and uric acid [J].
Li, Xingnan ;
Franke, Adrian A. .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (10) :853-856
[10]   Rapid prototyping of paper-based microfluidics with wax for low-cost, portable bioassay [J].
Lu, Yao ;
Shi, Weiwei ;
Jiang, Lei ;
Qin, Jianhua ;
Lin, Bingcheng .
ELECTROPHORESIS, 2009, 30 (09) :1497-1500