Exploratory Study of the Prevalence and Clinical Significance of Tobramycin-Mediated Biofilm Induction in Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients

被引：25

作者：

Elliott, Danielle ^{[2
,3
]}

Burns, Jane L. ^{[1
]}

Hoffman, Lucas R. ^{[1
]}

机构：

[1] Univ Washington, Dept Pediat, Seattle, WA 98105 USA

[2] Univ Washington, Dept Biochem, Seattle, WA 98105 USA

[3] Univ Washington, Dept Mol Cellular & Dev Biol, Seattle, WA 98105 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2010年 / 54卷 / 07期

关键词：

D O I：

10.1128/AAC.00102-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Exposure to aminoglycoside antibiotics induces biofilm formation by a laboratory strain of the cystic fibrosis (CF) pathogen Pseudomonas aeruginosa. Here, we detected this effect among about half of the clinical isolates from CF patients in a cross-sectional collection, suggesting that biofilm induction may represent a common mechanism of inducible aminoglycoside resistance in CF infections. This induction always occurred at the same tobramycin concentration regardless of MIC, suggesting that the mechanisms of killing and induction may be separable.

引用

收藏

页码：3024 / 3026

页数：3

相关论文

共 7 条

[1]

ELLIOTT D, 2009, 5 AM SOC MICR C BIOF

[2] Duration of treatment effect after tobramycin solution for inhalation in young children with cystic fibrosis [J].

Gibson, Ronald L. ;

Emerson, Julia ;

Mayer-Hamblett, Nicole ;

Burns, Jane L. ;

McNamara, Sharon ;

Accurso, Frank J. ;

Konstan, Michael W. ;

Chatfield, Barbara A. ;

Retsch-Bogart, George ;

Waltz, David A. ;

Acton, James ;

Zeitlin, Pamela ;

Hiatt, Peter ;

Moss, Richard ;

Williams, Judy ;

Ramsey, Bonnie W. ;

Grp, Tobramycin In Young Children Study .

PEDIATRIC PULMONOLOGY, 2007, 42 (07) :610-623

[3] Aminoglycoside antibiotics induce bacterial biofilm formation [J].

Hoffman, LR ;

D'Argenio, DA ;

MacCoss, MJ ;

Zhang, ZY ;

Jones, RA ;

Miller, SI .

NATURE, 2005, 436 (7054) :1171-1175

[4] Pseudomonas aeruginosa lasR mutants are associated with cystic fibrosis lung disease progression [J].

Hoffman, Lucas R. ;

Kulasekara, Hemantha D. ;

Emerson, Julia ;

Houston, Laura S. ;

Burns, Jane L. ;

Ramsey, Bonnie W. ;

Miller, Samuel I. .

JOURNAL OF CYSTIC FIBROSIS, 2009, 8 (01) :66-70

[5] Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3′-5′)-cyclic-GMP in virulence [J].

Kulesekara, H ;

Lee, V ;

Brencic, A ;

Liberati, N ;

Urbach, J ;

Miyata, S ;

Lee, DG ;

Neely, AN ;

Hyodo, M ;

Hayakawa, Y ;

Ausubel, FM ;

Lory, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (08) :2839-2844

[6] Aminoglycoside-resistance mechanisms for cystic fibrosis Pseudomonas aeruginosa isolates are unchanged by long-term, intermittent, inhaled tobramycin treatment [J].

MacLeod, DL ;

Nelson, LE ;

Shawar, RM ;

Lin, BB ;

Lockwood, LG ;

Dirks, JE ;

Miller, GH ;

Burns, JL ;

Garber, RL .

JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (03) :1180-1184

[7] Clinically feasible biofilm susceptibility assay for isolates of Pseudomonas aeruginosa from patients with cystic fibrosis [J].

Moskowitz, SM ;

Foster, JM ;

Emerson, J ;

Burns, JL .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) :1915-1922