Type II isopentenyl diphosphate isomerase from Synechocystis sp strain PCC 6803

被引：31

作者：

Barkley, SJ ^{[1
]}

Desai, SB ^{[1
]}

Poulter, CD ^{[1
]}

机构：

[1] Univ Utah, Dept Chem, Salt Lake City, UT 84112 USA

来源：

JOURNAL OF BACTERIOLOGY | 2004年 / 186卷 / 23期

关键词：

D O I：

10.1128/JB.186.23.8156-8158.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His(6)-tagged protein was produced in Escherichia coli and purified by Ni2+ chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg2+ for activity; K-m(IPP) was 52 muM, and k(cat)(IPP) was 0.23 s(-1).

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页码：8156 / 8158

页数：3

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