Novel potentiometric immunosensor for the detection of diphtheria antigen based on colloidal gold and polyvinyl butyral as matrixes

被引：51

作者：

Tang, DP ^{[1
]}

Yuan, R ^{[1
]}

Chai, YQ ^{[1
]}

Zhong, X ^{[1
]}

Liu, Y ^{[1
]}

Dai, JY ^{[1
]}

机构：

[1] SW China Normal Univ, Coll Chem & Chem Engn, Chong Qing Key Lab Analyt Chem, Chongqing 400715, Peoples R China

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2004年 / 22卷 / 01期

基金：

中国国家自然科学基金;

关键词：

potentiometric immunosensor; diphtheria; colloidal gold; polyvinyl butyral;

D O I：

10.1016/j.bej.2004.08.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel potentiometric immunosensor for the detection of diphtheria antigen (Diph) has been developed by means of self-assembly to immobilize diphtheria antibody (anti-Diph) on a platinum electrode based on colloidal gold (Au) and polyvinyl butyral (PVB) as matrixes. The modification procedure was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The interaction of antigen with grafted antibody recognition layers was carried out by soaking the modified electrode into a phosphate buffer solution at pH 7.0 with various concentrations of antigen. The detection is based on the change in the electric potential before and after the antigen-antibody reaction. Tests relating to the detection of diphtheria antigen demonstrate that the potentiometric immunosensor exhibits high sensitivity, wide linear range from 4.4 to 960 ng mL(-1) with a detection limit of 2.4 ng mL(-1), rapid response and long-term stability. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：43 / 49

页数：7

共 28 条

[1] REVERSIBLE IMMOBILIZATION OF AN ANTIBODY WITH A THIOL-SUBSTITUTED SORBENT - APPLICATION TO ENZYME IMMUNOASSAYS [J].