Protein-protein interaction between the RVS161 and RVS167 gene products of Saccharomyces cerevisiae

被引：48

作者：

Navarro, P ^{[1
]}

Durrens, P ^{[1
]}

Aigle, M ^{[1
]}

机构：

[1] CNRS, IBGC, Lab Biol Cellulaire Levures, F-33077 Bordeaux, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1997年 / 1343卷 / 02期

关键词：

RVS; two-hybrid system; cytoskeleton; (Saccharomyces cerevisiae);

D O I：

10.1016/S0167-4838(97)00108-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As previous studies indicated that the RVS161 and RVS167 gene products of Saccharomyces cerevisiae seem to be involved in the same cellular function, we considered the possibility of a complex between the proteins encoded by these two genes. Using the two hybrid system, we have shown that Rvs167p interacts with Rvs161p, through its N-terminal domain which contains predicted coiled-coil structures. Moreover, if a tagged Rvs167p protein was immune adsorbed under non-denaturing conditions, it brought along the Rvs161p protein. These results confirmed the hypothesis of an in vivo complex between the two proteins. (C) 1997 Elsevier Science B.V.

引用

页码：187 / 192

页数：6

共 31 条

[1] DEFINING PROTEIN INTERACTIONS WITH YEAST ACTIN IN-VIVO
AMBERG, DC
BASART, E
BOTSTEIN, D
[J]. NATURE STRUCTURAL BIOLOGY, 1995, 2 (01): : 28 - 35
[2] ALTERATION OF A YEAST SH3 PROTEIN LEADS TO CONDITIONAL VIABILITY WITH DEFECTS IN CYTOSKELETAL AND BUDDING PATTERNS
BAUER, F
URDACI, M
AIGLE, M
CROUZET, M
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (08) : 5070 - 5084
[3] SYNTHETIC LETHALITY WITH FIBRILLARIN IDENTIFIES NOP77P, A NUCLEOLAR PROTEIN REQUIRED FOR PRE-RIBOSOMAL-RNA PROCESSING AND MODIFICATION
BERGES, T
PETFALSKI, E
TOLLERVEY, D
HURT, EC
[J]. EMBO JOURNAL, 1994, 13 (13) : 3136 - 3148
[4] A FAMILY OF LOW AND HIGH COPY REPLICATIVE, INTEGRATIVE AND SINGLE-STRANDED SACCHAROMYCES-CEREVISIAE ESCHERICHIA-COLI SHUTTLE VECTORS
BONNEAUD, N
OZIERKALOGEROPOULOS, O
LI, GY
LABOUESSE, M
MINVIELLESEBASTIA, L
LACROUTE, F
[J]. YEAST, 1991, 7 (06) : 609 - 615
[5] PROTEIN-INTERACTION CLONING IN YEAST - IDENTIFICATION OF MAMMALIAN PROTEINS THAT REACT WITH THE LEUCINE ZIPPER OF JUN
CHEVRAY, PM
NATHANS, D
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (13) : 5789 - 5793
[6] THE 2-HYBRID SYSTEM - A METHOD TO IDENTIFY AND CLONE GENES FOR PROTEINS THAT INTERACT WITH A PROTEIN OF INTEREST
CHIEN, CT
BARTEL, PL
STERNGLANZ, R
FIELDS, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) : 9578 - 9582
[7] IDENTIFICATION OF A PROTEIN THAT BINDS TO THE SH3 REGION OF ABI AND IS SIMILAR TO BCR AND GAP-RHO
CICCHETTI, P
MAYER, BJ
THIEL, G
BALTIMORE, D
[J]. SCIENCE, 1992, 257 (5071) : 803 - 806
[8] YEAST MUTANT AFFECTED FOR VIABILITY UPON NUTRIENT STARVATION - CHARACTERIZATION AND CLONING OF THE RVS161 GENE
CROUZET, M
URDACI, M
DULAU, L
AIGLE, M
[J]. YEAST, 1991, 7 (07) : 727 - 743
[9] YEAST MUTANTS AFFECTED IN VIABILITY UPON STARVATION HAVE A MODIFIED PHOSPHOLIPID-COMPOSITION
DESFARGES, L
DURRENS, P
JUGUELIN, H
CASSAGNE, C
BONNEU, M
AIGLE, M
[J]. YEAST, 1993, 9 (03) : 267 - 277
[10] HOMOLOGY OF A YEAST ACTIN-BINDING PROTEIN TO SIGNAL TRANSDUCTION PROTEINS AND MYOSIN-I
DRUBIN, DG
MULHOLLAND, J
ZHU, ZM
BOTSTEIN, D
[J]. NATURE, 1990, 343 (6255) : 288 - 290

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