Molecular Cloning and Expression Analysis of Bat Toll-Like Receptors 3, 7 and 9

被引：22

作者：

Iha, Koichiro ^{[1
]}

Omatsu, Tsutomu ^{[3
]}

Watanabe, Shumpei ^{[2
]}

Ueda, Naoya ^{[1
]}

Taniguchi, Satoshi ^{[1
]}

Fujii, Hikaru ^{[2
]}

Ishii, Yoshiyuki ^{[1
]}

Kyuwa, Shigeru ^{[1
]}

Akashi, Hiroomi ^{[2
]}

Yoshikawa, Yasuhiro ^{[1
]}

机构：

[1] Univ Tokyo, Dept Biomed Sci, Bunkyo Ku, Tokyo 1138657, Japan

[2] Univ Tokyo, Dept Vet Microbiol, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[3] Natl Inst Infect Dis, Lab Vector Borne Viruses, Shinjuku Ku, Tokyo 1628650, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2010年 / 72卷 / 02期

关键词：

chiroptera; expression analysis; molecular cloning; sequencing; toll-like receptor; VIRUS-INFECTION; RECOGNITION; RESERVOIR;

D O I：

10.1292/jvms.09-0050

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

In this study, cDNA of Toll-like receptors (TLR) 3, 7 and 9 were synthesized and completely sequenced. The coding regions of cDNA for bat TLR3. TLR7 and TLR9 were 2,718, 3,150 and 3,090 bp in length, respectively. The open reading frames encoded 905, 1,049 and 1,029 amino acids for TLR3, TLR7 and TLR9, respectively. The nucleotide sequences, predicted amino acid sequences and predicted domain structures of the three bat TLRs had high homology with those of other mammals. In addition, the expression profiles of each TLR in main organs were analyzed. Expression of TLR3 was highest in the liver, whereas the expressions of TLR7 and TLR9 were highest in the spleen.

引用

页码：217 / 220

页数：4

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