Mismatched appositions of presynaptic and postsynaptic components in isolated hippocampal neurons

被引:111
作者
Rao, A
Cha, EM
Craig, AM
机构
[1] Washington Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63110 USA
[2] Univ Illinois, Dept Cell & Struct Biol, Urbana, IL 61801 USA
关键词
synaptogenesis; hippocampus; neuron culture; glutamate receptor; GABA receptor; PSD-95; gephyrin; autapse;
D O I
10.1523/JNEUROSCI.20-22-08344.2000
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
To determine whether presynaptic input is necessary for postsynaptic differentiation, we isolated hippocampal neurons in microisland culture and thus deprived pyramidal cells of GABA input and GABAergic neurons of glutamate input. We find that glutamate input is necessary for clustering the AMPA-type glutamate receptor but not for clustering the NMDA receptor or the associated PSD-95 family scaffold in GABAergic cells; GABA input is not necessary for clustering the GABA(A) receptor or gephyrin in pyramidal cells. Isolated neurons showed a surprising mismatch of presynaptic and postsynaptic components. For example, in isolated pyramidal neurons, although GABA(A) receptor clusters covered <4% of the dendritic surface and presynaptic boutons covered <12%, a full two-thirds of the GABA(A) receptor clusters were localized inappropriately opposite the non-GABAergic, presumed glutamatergic, terminals. Furthermore, inhibitory and excitatory postsynaptic components were segregated into separate clusters in isolated cells and apposed to separate boutons of a single axon. Thus, GABAA receptors were clustered opposite some terminals, whereas NMDA receptors were clustered opposite other terminals of a single axon. These results suggest the involvement of a synaptogenic signal common to glutamate and GABA synapses that permits experimentally induced mismatching of presynaptic and postsynaptic components in isolated neurons, as well as a second specificity-conferring signal that mediates appropriate matching in mixed cultures.
引用
收藏
页码:8344 / 8353
页数:10
相关论文
共 67 条
  • [1] Assembly of presynaptic active zones from cytoplasmic transport packets
    Ahmari, SE
    Buchanan, J
    Smith, SJ
    [J]. NATURE NEUROSCIENCE, 2000, 3 (05) : 445 - 451
  • [2] Distribution of neurotransmitter secretion in growing axons
    Antonov, I
    Chang, S
    Zakharenko, S
    Popov, SV
    [J]. NEUROSCIENCE, 1999, 90 (03) : 975 - 984
  • [3] Apperson ML, 1996, J NEUROSCI, V16, P6839
  • [4] EXCITATORY AND INHIBITORY AUTAPTIC CURRENTS IN ISOLATED HIPPOCAMPAL-NEURONS MAINTAINED IN CELL-CULTURE
    BEKKERS, JM
    STEVENS, CF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) : 7834 - 7838
  • [5] Benson DL, 1998, J NEUROSCI, V18, P6892
  • [6] THE FORMATION AND MATURATION OF SYNAPSES IN THE VISUAL-CORTEX OF THE RAT .2. QUANTITATIVE-ANALYSIS
    BLUE, ME
    PARNAVELAS, JG
    [J]. JOURNAL OF NEUROCYTOLOGY, 1983, 12 (04): : 697 - 712
  • [7] A synaptic Ras-GTPase activating protein (p135 SynGAP) inhibited by CaM kinase II
    Chen, HJ
    Rojas-Soto, M
    Oguni, A
    Kennedy, MB
    [J]. NEURON, 1998, 20 (05) : 895 - 904
  • [8] THE DISTRIBUTION OF GLUTAMATE RECEPTORS IN CULTURED RAT HIPPOCAMPAL-NEURONS - POSTSYNAPTIC CLUSTERING OF AMPA-SELECTIVE SUBUNITS
    CRAIG, AM
    BLACKSTONE, CD
    HUGANIR, RL
    BANKER, G
    [J]. NEURON, 1993, 10 (06) : 1055 - 1068
  • [9] Craig AM, 1996, J NEUROSCI, V16, P3166
  • [10] SELECTIVE CLUSTERING OF GLUTAMATE AND GAMMA-AMINOBUTYRIC-ACID RECEPTORS OPPOSITE TERMINALS RELEASING THE CORRESPONDING NEUROTRANSMITTERS
    CRAIG, AM
    BLACKSTONE, CD
    HUGANIR, RL
    BANKER, G
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) : 12373 - 12377