Monitoring activity of living cells entrapped in a membrane-sealed microcapillary with scanning electrochemical microscopy

被引：5

作者：

Skiku, H

Shiraishi, T

Ohya, H

Matsue, T

Abe, H

Hoshi, H

Kobayashi, M

机构：

[1] Japan Sci & Technol Corp, Reg Joint Res Project Yamagata Prefecture, Yamagata 9902473, Japan

[2] Inst Life Support Technol, Yamagata 9902473, Japan

[3] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Aoba Ku, Sendai, Miyagi 9808579, Japan

[4] Res Inst Funct Peptides, Yamagata 9900823, Japan

[5] Yamagata Agr Res & Training Ctr, Shinjo 9960041, Japan

来源：

ELECTROCHEMISTRY | 2000年 / 68卷 / 11期

关键词：

oxygen electrode; living cell; scanning electrochemical microscopy; small volume analysis;

D O I：

10.5796/electrochemistry.68.890

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

The respiration- or photosynthesis-induced change of oxygen concentration was monitored with scanning electrochemical microscopy (SECM). Cell suspension was put into a glass capillary tube and then one edge was sealed with an oxygen permeable membrane. The total volume of the cell suspension in the capillary was as small as 10 muL. The edge of the sealed capillary was imaged with SECM based on oxygen reduction current. The images indicated the respiration activity as low as 2.0 x 10(3) HeLa-S3 cells. This method was also applied far imaging the respiration and photosynthesis activities of a single alga (Bryopsis plumosa) protoplast.

引用

页码：890 / 892

页数：3

共 16 条

[1] SCANNING ELECTROCHEMICAL MICROSCOPY - INTRODUCTION AND PRINCIPLES [J].