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Chordin is required for the Spemann organizer transplantation phenomenon in Xenopus embryos

被引：112
作者：
Oelgeschläger, M
Kuroda, H
Reversade, B
De Robertis, EM [1 ]
机构：
[1] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Dept Biol Chem, Los Angeles, CA 90095 USA
来源：
DEVELOPMENTAL CELL | 2003年 / 4卷 / 02期
关键词：
D O I：
10.1016/S1534-5807(02)00404-5
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin production. Embryos developed with moderately reduced dorsoanterior structures and expanded ventroposterior tissues, phenocopying the zebrafish chordino mutant. A strong requirement for Chordin in dorsal development was revealed by experimental manipulations. First, dorsalization by lithium chloride treatment was completely blocked by Chd-MO. Second, Chd-MO inhibited elongation and muscle differentiation in Activin-treated animal caps. Third, Chd-MO completely blocked the induction of the central nervous system (CNS), somites, and notochord by organizer tissue transplanted to the ventral side of host embryos. Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation.
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收藏
页码：219 / 230
页数：12
相关论文
共 47 条
[1]   Chordin-like CR domains and the regulation of evolutionarily conserved extracellular signaling systems [J].
Abreu, JG ;
Coffinier, C ;
Larraín, J ;
Oelgeschläger, M ;
De Robertis, EM .
GENE, 2002, 287 (1-2) :39-47
[2]   The organizer factors Chordin and Noggin are required for mouse forebrain development [J].
Bachiller, D ;
Klingensmith, J ;
Kemp, C ;
Belo, JA ;
Anderson, RM ;
May, SR ;
McMahon, JA ;
McMahon, AP ;
Harland, RM ;
Rossant, J ;
De Robertis, EM .
NATURE, 2000, 403 (6770) :658-661
[3]   Wnt signaling in Xenopus embryos inhibits Bmp4 expression and activates neural development [J].
Baker, JC ;
Beddington, RSP ;
Harland, RM .
GENES & DEVELOPMENT, 1999, 13 (23) :3149-3159
[4]   The role of BMP signaling in outgrowth and patterning of the Xenopus tail bud [J].
Beck, CW ;
Whitman, M ;
Slack, JMW .
DEVELOPMENTAL BIOLOGY, 2001, 238 (02) :303-314
[5]   Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer [J].
Bouwmeester, T ;
Kim, SH ;
Sasai, Y ;
Lu, B ;
DeRobertis, EM .
NATURE, 1996, 382 (6592) :595-601
[6]   Twisted gastrulation can function as a BMP antagonist [J].
Chang, CB ;
Holtzman, DA ;
Chau, S ;
Chickering, T ;
Woolf, EA ;
Holmgren, LM ;
Bodorova, J ;
Gearing, DP ;
Holmes, WE ;
Brivanlou, AH .
NATURE, 2001, 410 (6827) :483-487
[7]  
DALE L, 1987, DEVELOPMENT, V100, P279
[8]   The establishment of Spemann's organizer and patterning of the vertebrate embryo [J].
De Robertis, EM ;
Larraín, J ;
Oelgeschläger, M ;
Wessely, O .
NATURE REVIEWS GENETICS, 2000, 1 (03) :171-181
[9]  
DEROBERTIS EM, 2001, ITN J DEV BIOL, V45
[10]   The interpretation of position in a morphogen gradient as revealed by occupancy of activin receptors [J].
Dyson, S ;
Gurdon, JB .
CELL, 1998, 93 (04) :557-568
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