Ionic strength effect on adsorption of cellobiohydrolases I and II on microcrystalline cellulose

被引：15

作者：

Kim, DW ^{[1
]}

Hong, YG ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Coll Nat Sci, Dept Chem, Cheongju 361763, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 16期

关键词：

adsorption; cellulase; cellulose; ionic strength; thermodynamics;

D O I：

10.1023/A:1005663316018

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cellobiohydrolase I (CBH I) has a higher adsorption affinity (K-ad) and tightness (-Delta H-a) for Avicel than cellobiohydrolase II (CBH II). The adsorption processes of CBH I and II were exothermic, and the degree of exothermy were larger with the increasing ionic strength. Entropy change of CBH I was larger than CBH II with increasing ionic strength. CBH I was more effective than CBH II for binding at a given ionic strength.

引用

页码：1337 / 1342

页数：6

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