Yacon (Polymnia sanchifolia) extract as a substrate to produce inulinase by Kluyveromyces marxianus var. bulgaricus

被引:63
作者
Cazetta, ML
Martins, PMM
Monti, R
Contiero, J
机构
[1] UNESP, Dept Bioquim & Microbiol, Inst Biociencias, BR-13506900 Rio Claro, Brazil
[2] UNESP, Fac Ciencias Farmaceut, Dept Alimentos & Nutr, BR-13506900 Rio Claro, Brazil
关键词
Kluyveromyces marxianus var. bulgaricus; yacon; inulinase; inulin; fructooligosaccharides; Polymnia sanchifolia;
D O I
10.1016/j.jfoodeng.2004.03.022
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
The inulinase production by yeast K marxianus var. bulgaricus growing in yacon extract was investigated. The microorganism showed good development in yacon, higher enzymatic activities were achieved at 30% and 40% (v/v) of extract. The cultivation temperature (20, 25, 30, 35, 40 degreesC) neither influenced the growth or the enzymatic activity. The optimum cultivation pH was 3.5. The highest activity was observed at 60 degreesC and pH 4.0. At temperature of 55 C and 60 C occurred sharp decrease in the enzyme activity. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:301 / 305
页数:5
相关论文
共 22 条
[1]  
[Anonymous], PROCESS BIOCH, DOI DOI 10.1016/S0032-9592(98)00140-X
[2]   Hypoglycemic effect of the water extract of Smallantus sonchifolius (yacon) leaves in normal and diabetic rats [J].
Aybar, MJ ;
Riera, ANS ;
Grau, A ;
Sánchez, SS .
JOURNAL OF ETHNOPHARMACOLOGY, 2001, 74 (02) :125-132
[3]  
CAZETTA ML, 2002, 1O ENC BIOL CONS REG
[4]   KLUYVEROMYCES-MARXIANUS CDBB-L-278 - A WILD INULINASE HYPERPRODUCING STRAIN [J].
CRUZGUERRERO, A ;
GARCIAPENA, I ;
BARZANA, E ;
GARCIAGARIBAY, M ;
GOMEZRUIZ, L .
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1995, 80 (02) :159-163
[5]   Sucrose hydrolysis by thermostable immobilized inulinases from Aspergillus ficuum [J].
Ettalibi, M ;
Baratti, JC .
ENZYME AND MICROBIAL TECHNOLOGY, 2001, 28 (7-8) :596-601
[6]  
Kalil S.J., 1999, P 2 C ENG PROC MERCO
[7]   Production, purification and characterization of an extracellular inulinase from Kluyveromyces marxianus var. bulgaricus [J].
Kushi, RT ;
Monti, R ;
Contiero, J .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2000, 25 (02) :63-69
[8]  
MANZONI M, 1988, LEBENSM WISS TECHNOL, V21, P271
[9]  
MANZONI M, 1990, KLUYVEROMYCES CICERI, V24, P236
[10]  
MILLER LG, 1959, ANAL CHEM, V31, P3