Comparative immunochemical characterization of polyclonal and monoclonal antibodies to aflatoxin B1

被引：2

作者：

Burkin, MA

Yakovleva, IV

Sviridov, VV

Burkin, AA

Kononenko, GP

Soboleva, NA

机构：

[1] Russian Acad Sci, Mechnikov Inst Vaccines & Sera, Moscow 103064, Russia

[2] Russian Acad Agr Sci, All Russia Inst Vet Sanitat, Moscow 123022, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2000年 / 36卷 / 05期

关键词：

Apply Biochemistry; Aflatoxin; Mold Fungus; ELISA Condition; Immune Reagent;

D O I：

10.1007/BF02731897

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Four hybrid clones (MM-(AB(1))-I, MM-(AB(1))-2, MM-(AB(1))-3, and MM-(AB(1))-4) were obtained by hybridoma technology involving the immunization of BALB/c mice with a BSA conjugate of aflatoxin B-1 carboxymethyloxime derivative. Antibodies produced by these clones varied in their ability to recognize the aflatoxin B-1 analogues. The sensitivity of enzyme immunoassay based on all monoclonal antibodies was higher compared to analysis based on polyclonal rabbit antibodies (0.1 and 0.4 ng/ml, respectively).

引用

页码：496 / 501

页数：6

共 11 条

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[2]

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