Silver nanowires-based signal amplification for CdSe quantum dots electrochemiluminescence immunoassay

被引：33

作者：

Huang, Tingyu ^{[1
]}

Meng, Qingmin ^{[2
]}

Jie, Guifen ^{[2
]}

机构：

[1] Linyi Univ, Linyi 276000, Peoples R China

[2] Qingdao Univ Sci & Technol, Minist Educ, Key Lab Sensor Anal Tumor Marker, Qingdao 266042, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 66卷

基金：

中国国家自然科学基金;

关键词：

Silver-cysteine; Hybrid nanowires; SCNWs-QDs; Electrochemiluminescence; Immunoassay; ELECTROGENERATED CHEMILUMINESCENCE; ENHANCED ELECTROCHEMILUMINESCENCE; NANOMATERIALS; IMMUNOSENSOR; CONFORMATION;

D O I：

10.1016/j.bios.2014.11.011

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel silver-cysteine hybrid nanowires (SCNWs) with many reactive carboxyl and amine groups were prepared, which enable them to be used as idea signal amplifying labels in bioassays. A large number of CdSe quantum dots (QDs) were loaded on the SCNWs to develop amplified SCNWs-QDs electro-chemiluminescence (ECL) signal probe. The PAMAM dendrimer-SCNWs nanohybrids covered on the electrode constructed an effective antibody immobilization matrix and made the immobilized biomolecules hold high stability and bioactivity. Based on the specific sandwich immunoreaction strategy, the detection antibody (Ab(2))-SCNWs-QDs ECL signal probe was applied to the sensitive signal-on ECL immunoassay of human IgG. The SCNWs-QDs ECL not only opens promising new ECL emitting species, but also promotes the development of novel ECL signal-transition platforms for biosensing devices. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：84 / 88

页数：5

共 23 条

[1] Nanomaterials for rechargeable lithium batteries [J].