Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxin

被引：323

作者：

Tu, JM

Zhang, GA

Datta, K

Xu, CG

He, YQ

Zhang, QF

Khush, GS

Datta, SK

机构：

[1] Int Rice Res Inst, Plant Breeding Genet & Biochem Div, Makati City 1271, Philippines

[2] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

来源：

NATURE BIOTECHNOLOGY | 2000年 / 18卷 / 10期

关键词：

hybrid rice; fusion Bt protein; transgenic indica restorer line; field performance; pest control;

D O I：

10.1038/80310

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here we describe development of transgenic elite rice lines expressing a St fusion gene derived from cryIA(b) and cryIA(c) under the control of rice acfinI promoter. The lines used in the study were indica CMS restorer line of Minghui 63 and its derived hybrid rice Shanyou 63, The level of St fusion protein CryIA(b)/CryIA(c) detected in Minghui 63 (T51-1) plants was 20 ng/mg soluble protein. The St Shanyou 63 was field-tested in natural and repeated heavy manual infestation of two lepidopteran insects, leaffolder and yellow stem borer. The transgenic hybrid plants showed high protection against both insect pests without reduced yield.

引用

页码：1101 / 1104

页数：4

共 37 条

[1] CHARACTERIZED FULL-LENGTH AND TRUNCATED PLASMID CLONES OF THE CRYSTAL PROTEIN OF BACILLUS-THURINGIENSIS SUBSP KURSTAKI HD-73 AND THEIR TOXICITY TO MANDUCA-SEXTA [J].