Characterization of microsatellite loci in the Kaka, Nestor meridionalis

被引:8
作者
Sainsbury, JP [1 ]
Macavoy, ES [1 ]
Chambers, GK [1 ]
机构
[1] Victoria Univ Wellington, Sch Biol Sci, Inst Mol Systemat, Wellington, New Zealand
来源
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 04期
关键词
conservation; microsatellite; Nestor meridionalis; parrot; population genetics;
D O I
10.1111/j.1471-8286.2004.00722.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Eight microsatellite loci were characterized from the Kaka (Nestor meridionalis), a New Zealand parrot, using a polymerase chain reaction-based isolation technique. Locus-specific primers were used to genotype nine Kaka populations and tested on 25 other species of parrot. The number of alleles observed within Kaka ranged from one to 16 in the 12-126 individuals screened and two loci exhibited greater than 60% heterozygosity. Furthermore, these primers are likely to be useful in population-level studies of two other New Zealand parrots.
引用
收藏
页码:623 / 625
页数:3
相关论文
共 9 条
[1]  
Boon W. M., 2000, THESIS VICTORIA U WE
[2]   Characterization of microsatellite loci from the socially monogamous lizard Tiliqua rugosa using a PCR-based isolation technique [J].
Cooper, SJB ;
Bull, CM ;
Gardner, MG .
MOLECULAR ECOLOGY, 1997, 6 (08) :793-795
[3]  
GRIST SA, 1993, BIOTECHNIQUES, V15, P304
[4]   PERFORMING THE EXACT TEST OF HARDY-WEINBERG PROPORTION FOR MULTIPLE ALLELES [J].
GUO, SW ;
THOMPSON, EA .
BIOMETRICS, 1992, 48 (02) :361-372
[5]  
HUGGINS J, 2001, THESIS VICTORIA U WE
[6]  
MOORHOUSE RJ, 1998, RES PLAN KAKA
[7]  
Primmer CR, 1997, MOL ECOL, V6, P101
[8]   GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].
RAYMOND, M ;
ROUSSET, F .
JOURNAL OF HEREDITY, 1995, 86 (03) :248-249
[9]  
Yeh F. C., 1999, POPGENE VERSION 1 3