Folate synthesis in plants:: the last step of the p-aminobenzoate branch is catalyzed by a plastidial aminodeoxychorismate lyase

被引:70
作者
Basset, GJC
Ravanel, S
Quinlivan, EP
White, R
Giovannoni, JJ
Rébeillé, F
Nichols, BP
Shinozaki, K
Seki, M
Gregory, JF
Hanson, AD [1 ]
机构
[1] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA
[2] Univ Grenoble 1, CNRS, CEA Grenoble,INRA, Physiol Cellulaire Vegetale Lab, F-38054 Grenoble 9, France
[3] Univ Florida, Dept Food Sci & Human Nutr, Gainesville, FL 32611 USA
[4] ARS, USDA, Ithaca, NY 14853 USA
[5] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
[6] Univ Illinois, Dept Biol Sci, Chicago, IL 60607 USA
[7] RIKEN Tsukuba Inst, Plant Mol Biol Lab, Tsukuba, Ibaraki 3050074, Japan
[8] RIKEN Genom Sci Ctr, Plant Mutat Explorat Team, Plant Funct Genom Res Grp, Kanagawa 2300045, Japan
关键词
p-aminobenzoate; aminodeoxychorismate lyase; folate biosynthesis; plastid targeting; Arabidopsis; tomato;
D O I
10.1111/j.1365-313X.2004.02231.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In plants, the last step in the synthesis of p-aminobenzoate (PABA) moiety of folate remains to be elucidated. In Escherichia coli, this step is catalyzed by the PabC protein, a beta-lyase that converts 4-amino-4-deoxychorismate (ADC) - the reaction product of the PabA and PabB enzymes - to PABA and pyruvate. So far, the only known plant enzyme involved in PABA synthesis is ADC synthase, which has fused domains homologous to E. coli PabA and PabB and is located in plastids. ADC synthase has no lyase activity, implying that plants have a separate ADC lyase. No such lyase is known in any eukaryote. Genomic and phylogenetic approaches identified Arabidopsis and tomato cDNAs encoding PabC homologs with putative chloroplast-targeting peptides. These cDNAs were shown to encode functional enzymes by complementation of an E. coli pabC mutant, and by demonstrating that the partially purified recombinant proteins convert ADC to PABA. Plant ADC lyase is active as dimer and is not feedback inhibited by physiologic concentrations of PABA, its glucose ester, or folates. The full-length Arabidopsis ADC lyase polypeptide was translocated into isolated pea chloroplasts and, when fused to green fluorescent protein, directed the passenger protein to Arabidopsis chloroplasts in transient expression experiments. These data indicate that ADC lyase, like ADC synthase, is present in plastids. As shown previously for the ADC synthase transcript, the level of ADC lyase mRNA in the pericarp of tomato fruit falls sharply as ripening advances, suggesting that the expression of these two enzymes is coregulated.
引用
收藏
页码:453 / 461
页数:9
相关论文
共 38 条
[1]   TRANSIENT TRANSFORMATION OF ARABIDOPSIS LEAF PROTOPLASTS - A VERSATILE EXPERIMENTAL SYSTEM TO STUDY GENE-EXPRESSION [J].
ABEL, S ;
THEOLOGIS, A .
PLANT JOURNAL, 1994, 5 (03) :421-427
[2]   SYNTHESIS OF THE SMALL SUBUNIT OF RIBULOSE-BISPHOSPHATE CARBOXYLASE FROM GENES CLONED INTO PLASMIDS CONTAINING THE SP6 PROMOTER [J].
ANDERSON, S ;
SMITH, SM .
BIOCHEMICAL JOURNAL, 1986, 240 (03) :709-715
[3]   USE OF THE TAC PROMOTER AND LACIQ FOR THE CONTROLLED EXPRESSION OF ZYMOMONAS-MOBILIS FERMENTATIVE GENES IN ESCHERICHIA-COLI AND ZYMOMONAS-MOBILIS [J].
ARFMAN, N ;
WORRELL, V ;
INGRAM, LO .
JOURNAL OF BACTERIOLOGY, 1992, 174 (22) :7370-7378
[4]   Folate synthesis in plants: The first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase I [J].
Basset, G ;
Quinlivan, EP ;
Ziemak, MJ ;
de la Garza, RD ;
Fischer, M ;
Schiffmann, S ;
Bacher, A ;
Gregory, JF ;
Hanson, AD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (19) :12489-12494
[5]   Foliate synthesis in plants:: The p-aminobenzoate branch is initiated by a bifunctional PabA-PabB protein that is targeted to plastids [J].
Basset, GJC ;
Quinlivan, EP ;
Ravanel, S ;
Rébeillé, F ;
Nichols, BP ;
Shinozaki, K ;
Seki, M ;
Adams-Phillips, LC ;
Giovannoni, JJ ;
Gregory, JF ;
Hanson, AD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (06) :1496-1501
[6]  
BASSET GJC, 2004, IN PRESS CROP SCI
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   Polyglutamylation of folate coenzymes is necessary for methionine biosynthesis and maintenance of intact mitochondrial genome in Saccharomyces cerevisiae [J].
Cherest, H ;
Thomas, D ;
Surdin-Kerjan, Y .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (19) :14056-14063
[9]   Engineered GFP as a vital reporter in plants [J].
Chiu, WL ;
Niwa, Y ;
Zeng, W ;
Hirano, T ;
Kobayashi, H ;
Sheen, J .
CURRENT BIOLOGY, 1996, 6 (03) :325-330
[10]   MULTIPLE PATHWAYS FOR PROTEIN-TRANSPORT INTO OR ACROSS THE THYLAKOID MEMBRANE [J].
CLINE, K ;
HENRY, R ;
LI, CJ ;
YUAN, JG .
EMBO JOURNAL, 1993, 12 (11) :4105-4114