Highly sensitive non-stripping gold nanoparticles-based chemiluminescent detection of DNA hybridization coupled to magnetic beads

被引：19

作者：

Cai, Sheng ^{[1
]}

Xin, Liang ^{[1
]}

Lau, Choiwan ^{[1
]}

Lu, Jianzhong ^{[1
]}

机构：

[1] Fudan Univ, Sch Pharm, Shanghai 201203, Peoples R China

来源：

ANALYST | 2010年 / 135卷 / 03期

基金：

中国国家自然科学基金;

关键词：

SEQUENCE-SPECIFIC DNA; NUCLEIC-ACID LABELS; ELECTROCHEMICAL DETECTION; ELECTROGENERATED CHEMILUMINESCENCE; AU NANOPARTICLES; ONE-STEP; PROBES; IMMUNOASSAY; TARGETS; TECHNOLOGY;

D O I：

10.1039/b927359j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have developed a new nanoparticle (NP)-based chemiluminescent (CL) method for the sensitive detection of DNA hybridization. The "sandwich-type'' DNA technique comprises NH2-functionalized capture DNA immobilized on magnetic beads that can hybridize with one end of the target DNA. The other end of the target is recognized by a reporter DNA probe labeled with Au NPs on its 3-terminus. The hybridization events were monitored by a simple and direct luminol-AgNO3 CL reaction with a detection limit of 0.5 pM through a non-stripping Au NPs catalysis. Moreover, the CL reaction of non-stripping Au NPs-luminol-AgNO3 offers the advantages of being simpler and faster as compared to previous stripping Ag NPs and CuS methods. This simple and easy-to-use technique holds great promise for clinical applications of DNA hybridization.

引用

页码：615 / 620

页数：6

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