Expression of P2X7 purinoceptors on human lymphocytes and monocytes:: evidence for nonfunctional P2X7 receptors

被引:183
作者
Gu, BJ
Zhang, WY
Bendall, LJ
Chessell, IP
Buell, GN
Wiley, JS
机构
[1] Univ Sydney, Nepean Hosp, Dept Med, Penrith, NSW 2750, Australia
[2] Univ Cambridge, Dept Pharmacol, Cambridge CB2 1QJ, England
[3] Geneva Inst Biomed Res, Dept Mol Biol, CH-1228 Geneva, Switzerland
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2000年 / 279卷 / 04期
关键词
extracellular adenosine 5 '-triphosphate; adenosine 5 '-triphosphate-induced ethidium uptake; monocyte P2X(7); lymphocyte P2X(7); B cell chronic lymphocytic leukemia;
D O I
10.1152/ajpcell.2000.279.4.C1189
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Lymphocytes from normal subjects and patients with B-chronic lymphocytic leukemia (B-CLL) show functional responses to extracellular ATP characteristic of the P2X(7) receptor (previously termed P2Z). These responses include opening of a cation-selective channel/pore that allows entry of the fluorescent dye ethidium and activation of a membrane metalloprotease that sheds the adhesion molecule L-selectin. The surface expression of P2X(7) receptors was measured in normal leucocytes, platelets, and B-CLL lymphocytes and correlated with their functional responses. Monocytes showed four- to fivefold greater expression of P2X(7) than B, T, and NK lymphocytes, whereas P2X(7) expression on neutrophils and platelets was weak. All cell types demonstrated abundant intracellular expression of this receptor. All 12 subjects with B-CLL expressed lymphocyte P2X(7) at about the same level as B lymphocytes from normal subjects. P2X(7) function, measured by ATP-induced uptake of ethidium, correlated closely with surface expression of this receptor in normal and B-CLL lymphocytes and monocytes (n = 47, r = 0.70; P< 0.0001). However, in three patients the ATP-induced uptake of ethidium into the malignant B lymphocytes was low or absent. The lack of P2X(7) function in these B lymphocytes was confirmed by the failure of ATP to induce Ba2+ uptake into their lymphocytes. This lack of function of the P2X(7) receptor resulted in a failure of ATP-induced shedding of L-selectin, an adhesion molecule that directs the recirculation of lymphocytes from blood into the lymph node.
引用
收藏
页码:C1189 / C1197
页数:9
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