Maize polyamine oxidase:: primary structure from protein and cDNA sequencing

The first complete amino acid sequence of a flavin-containing polyamine oxidase was solved by a combined approach of nucleotide and peptide sequence analysis, A cDNA of 1737 bp, isolated from maize seedlings by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends strategies, was cloned and its sequence determined, This cDNA contains information for a polypeptide chain of 500 amino acids, Its amino-terminal sequence shows the typical features of secretion signal peptides, The primary structure of the mature protein was independently confirmed by extensive amino acid sequencing. Structural relationships with flavin-containing monoamine oxidases are also discussed. (C) 1998 Federation of European Biochemical Societies.