PIG-B: A homemade monophasic cocktail for the extraction of RNA

被引：35

作者：

Weber, K ^{[1
]}

Bolander, ME ^{[1
]}

Sarkar, G ^{[1
]}

机构：

[1] Mayo Clin & Mayo Fdn, Dept Orthoped, Rochester, MN USA

来源：

MOLECULAR BIOTECHNOLOGY | 1998年 / 9卷 / 01期

关键词：

RNA extraction; reagent; RT-PCR amplification;

D O I：

10.1007/BF02752699

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An inexpensive monophasic reagent has been developed for the extraction of total RNA from cells or tissues. The main ingredients of the reagent are Phenol, Isoamyl alcohol, Guanidinium isothiocyanate, and Beta-mercaptoethanol (PIG-B). The quality and yield of RNA obtained by this reagent is at par with that obtained by TRIzol, an expensive but widely used monophasic reagent available commercially. The complete composition and method of preparation of PIG-B is provided to aid preparation of the reagent in the laboratory.

引用

页码：73 / 77

页数：5

共 6 条

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