Inhibition of tyrosine hydroxylase expression in α-synuclein-transfected dopaminergic neuronal cells

被引：126

作者：

Yu, S

Zuo, XH

Li, YH

Zhang, C

Zhou, M

Zhang, YA

Uéda, K

Chan, P

机构：

[1] Capital Univ Med Sci, Dept Neurobiol, Beijing 100053, Peoples R China

[2] Capital Univ Med Sci, Sino Japan Joint Lab Neurodegenerat Dis, Beijing Inst Geriatr, Xuanwu Hosp, Beijing 100053, Peoples R China

[3] Tokyo Inst Psychiat, Dept Neural Plast, Tokyo 1568585, Japan

来源：

NEUROSCIENCE LETTERS | 2004年 / 367卷 / 01期

基金：

中国国家自然科学基金;

关键词：

alpha-synuclein; tyrosine hydroxylase; Parkinson's disease; dopaminergic neuron; gene transfection;

D O I：

10.1016/j.neulet.2004.05.118

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

Although the aberrant expression of alpha-synuclein (alpha-Syn) is toxic to dopaminergic neurons, little is known about the correlation between the abnormality of alpha-Syn and the expression of tyrosine hydroxylase (TH), a rate-limiting enzyme for the synthesis of dopamine neurotransmitter. In this study, the MES23.5 rat dopaminergic cell line transfected with wild-type human alpha-Syn cDNA (halpha-Syn) construct was used to investigate the association between alpha-Syn and TH. Immunocytochemical staining and Western blot for TH showed that the TH expression was greatly decreased in halpha-Syn-transfected cells. Northern blot confirmed that the TH mRNA level was also dramatically reduced. Reduction of TH protein levels did not affect the growth and proliferation of the transfected cells. No apparent cell injury or death was observed. These results suggest that an abnormal expression of alpha-Syn may inhibit TH synthesis in dopaminergic cells. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：34 / 39

页数：6

共 37 条

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