Thrombocytopenia associated with apoptotic megakaryocytes in a viral haemorrhagic syndrome induced by a moderately virulent strain of African swine fever virus

被引:20
作者
Gómez-Villamandos, JC
Bautista, MJ
Carrasco, L
de Lara, FCM
Hervás, J
Wilkinson, PJ
Sierra, MA
机构
[1] Univ Cordoba, Fac Vet, Dept Anat & Anat Patol Comparadas, Cordoba 14005, Spain
[2] Inst Anim Hlth, Pirbright Lab, Surrey, England
关键词
D O I
10.1016/S0021-9975(98)80023-6
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
A viral haemorrhagic syndrome was induced in 14 pigs by inoculation with an African swine fever (ASF) virus strain of moderate virulence, to determine changes in megakaryocyte (MK) numbers and morphology and thus to assess the role of these cells in the thrombocytopenia characteristic of subacute ASF. The strain tested induced changes in the proportion of different types of MK (typical nucleated MKs, apoptotic MKs and immature MKs); it also caused subcellular lesions over the first 7 days post-inoculation (dpi). At 7 dpi, severe thrombocytopenia was observed. There was a statistically significant increase in apoptotic MK numbers. The MKs showed three stages in the course of the disease: a compensatory stage, represented by cytoplasmic projections, a hypermaturity stage, represented by apoptotic MKs, and a regenerative stage, represented by clusters of immature MKs. These changes, especially the presence of numerous apoptotic MKs, may explain the early and transitory thrombocytopenia detected in subacute ASF. The large number of apoptotic MKs observed may be associated with the accelerated maturation of these cells, resulting from the action of cytokines, or peripheral platelet consumption, or both. (C) 1998 W.B. Saunders Company Limited.
引用
收藏
页码:1 / 13
页数:13
相关论文
共 30 条
[1]  
BAGBY GC, 1987, BLOOD CELLS, V13, P147
[2]   PATHO-PHYSIOLOGY OF THROMBOCYTOPENIA ASSOCIATED WITH HIV INFECTION IN HOMOSEXUAL MEN - A PRELIMINARY-REPORT [J].
BALLEM, PJ ;
BELZBERG, A ;
DEVINE, D ;
DOUBROFF, P ;
MIKULASH, C ;
SPURSTON, B ;
CHAMBERS, H ;
SARTORI, C ;
BUSKARD, N .
BLUT, 1989, 59 (01) :111-114
[3]  
BAUER S, 1992, ARCH PATHOL LAB MED, V116, P1025
[4]   IMMUNOCHEMICAL ANALYSIS OF PLATELET AUTOANTIBODIES IN HIV-RELATED THROMBOCYTOPENIC PURPURA - A STUDY OF 68 PATIENTS [J].
BETTAIEB, A ;
OKSENHENDLER, E ;
FROMONT, P ;
DUEDARI, N ;
BIERLING, P .
BRITISH JOURNAL OF HAEMATOLOGY, 1989, 73 (02) :241-247
[5]   HISTOLOGIC-FINDINGS IN BONE-MARROW BIOPSIES OF PATIENTS WITH THROMBOCYTHEMIC CELL COUNTS [J].
BUHR, T ;
GEORGII, A ;
SCHUPPAN, O ;
AMOR, A ;
KALOUTSI, V .
ANNALS OF HEMATOLOGY, 1992, 64 (06) :286-291
[6]   Apoptosis in lymph nodes in acute African swine fever [J].
Carrasco, L ;
deLara, FCM ;
delasMulas, JM ;
GomezVillamandos, JC ;
Perez, J ;
Wilkinson, PJ ;
Sierra, MA .
JOURNAL OF COMPARATIVE PATHOLOGY, 1996, 115 (04) :415-428
[7]   THE FREQUENCY AND SIGNIFICANCE OF MEGAKARYOCYTIC EMPERIPOLESIS IN MYELOPROLIFERATIVE AND REACTIVE STATES [J].
CASHELL, AW ;
BUSS, DH .
ANNALS OF HEMATOLOGY, 1992, 64 (06) :273-276
[8]   A PLASMA INHIBITOR OF PLATELET-AGGREGATION IN PATIENTS WITH ARGENTINE HEMORRHAGIC-FEVER [J].
CUMMINS, D ;
MOLINAS, FC ;
LERER, G ;
MAIZTEGUI, JI ;
FAINT, R ;
MACHIN, SJ .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1990, 42 (05) :470-475
[9]  
GOMEZVILLAMANDO.JC, 1997, IN PRESS VET RES
[10]   A PATHOLOGICAL-STUDY OF THE PERISINUSOIDAL UNIT OF THE LIVER IN ACUTE AFRICAN SWINE FEVER [J].
GOMEZVILLAMANDOS, JC ;
HERVAS, J ;
MENDEZ, A ;
CARRASCO, L ;
VILLEDA, CJ ;
WILKINSON, PJ ;
SIERRA, MA .
RESEARCH IN VETERINARY SCIENCE, 1995, 59 (02) :146-151