Donor and Recipient Envs from Heterosexual Human Immunodeficiency Virus Subtype C Transmission Pairs Require High Receptor Levels for Entry

被引：48

作者：

Alexander, Melissa ^{[1
]}

Lynch, Rebecca ^{[1
]}

Mulenga, Joseph ^{[3
,4
]}

Allen, Susan ^{[2
]}

Derdeyn, Cynthia A. ^{[1
,2
]}

Hunter, Eric ^{[1
,2
]}

机构：

[1] Emory Univ, Emory Vaccine Ctr, Yerkes Natl Primate Res Ctr, Atlanta, GA 30329 USA

[2] Emory Univ, Dept Pathol & Lab Med, Atlanta, GA 30329 USA

[3] Zambia Emory HIV Res Project ZEHRP, Lusaka, Zambia

[4] Zambia Blood Transfus Serv, Lusaka, Zambia

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 08期

关键词：

GENETIC SUBTYPES; VARIABLE LOOPS; TYPE-1; NEUTRALIZATION; VARIANTS; HIV-1; CCR5; V2; GLYCOSYLATION; CD4;

D O I：

10.1128/JVI.02068-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Compact, glycan-restricted envelope (Env) glycoproteins are selected during heterosexual transmission of subtype C HIV-1. Donor and recipient glycoproteins (Envs) from six transmission pairs were evaluated for entry into HeLa cells expressing different levels of CD4 and CCR5. Donor and recipient Envs demonstrated efficient entry into cells expressing high levels of CD4 and CCR5, and entry declined as CCR5 levels decreased. Infectivity for all Envs was severely impaired in cells expressing low levels of CD4, even at the highest CCR5 levels. In 5/6 pairs, there was no significant difference in efficiency of receptor utilization between the donor and recipient Envs in these HeLa-derived cell lines. Thus, HIV-1 transmission does not appear to select for viruses that can preferentially utilize low levels of entry receptors.

引用

页码：4100 / 4104

页数：5

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