Interspecific gene flow as a component of risk assessment for transgenic Brassicas

被引：24

作者：

Chevre, AM ^{[1
]}

Eber, F ^{[1
]}

Baranger, A ^{[1
]}

Kerlan, MC ^{[1
]}

Barret, P ^{[1
]}

Festoc, G ^{[1
]}

Vallee, P ^{[1
]}

Renard, M ^{[1
]}

机构：

[1] INRA, Stn Ameliorat Plantes, F-35650 Le Rheu, France

来源：

INTERNATIONAL SYMPOSIUM ON BRASSICAS: NINTH CRUCIFER GENETICS WORKSHOP | 1996年 / 407期

关键词：

D O I：

10.17660/ActaHortic.1996.407.21

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Interspecific hybrids were produced between three diploid weeds (R. raphanistrum, RrRr, 2n=18, B. adpressa, AdAd, 2n=14 and S. arvensis, SarSar, 2n=18) and B. napus (AACC, 2n=38) either a transgenic herbicide resistant rapeseed, under controlled conditions (i.e. hand pollination and embryo rescue) or a male sterile rapeseed under field conditions. Per 100 pollinated flowers, for the first method, 0.0 (with S. arvensis as female parent) to 11.6 (with B. adpressa as male parent) embryos were obtained whereas the number of seeds ranged from 0.18 (with S. arvensis) to 23.8 (with R. raphanistrum) under open pollination. Most of the hybrids had the expected triploid structure (ACX) except some amphidiploids plants (AACCXX). The meiotic behaviour of the triploid hybrids revealed that recombination might occur preferentially between AC and Rr genomes. Seed set of Fl interspecific hybrids was in any case low under optimal conditions. In the advanced progenies of B. napus-R. raphanistrum hybrids, the chromosome number remained high as well as the percentage of rapeseed specific molecular markers; same observations were realized on B. napus-B. oleracea progenies. From these results, the possibility of gene flow between crucifer species is discussed.

引用

页码：169 / 179

页数：3