Lack of cross-reactivity of anti-DNA antibodies to ribonucleoproteins in a new commercial blot system for specific ANAs

被引：3

作者：

González, C ^{[1
]}

Pascual, MJ ^{[1
]}

González-Buitrago, JM ^{[1
]}

机构：

[1] Univ Hosp, Serv Bioquim, Salamanca 37007, Spain

来源：

AUTOIMMUNITY | 2000年 / 32卷 / 02期

关键词：

blotting; ELISA; anti-U(1)snRNP; anti-Sm;

D O I：

10.3109/08916930008994082

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Anti-DNA antibodies often exhibit cross-reactivity, It has been observed that anti-DNA antibodies cross-react with A and D snRNP proteins in an in-house developed Western blot assay but do not cross-react with native U1RNP in ELISA or immunoprecipitation experiments. We have analyzed the cross-reactivity of anti-DNA antibodies to snRNP A and D in a recently developed commercial blot assay (InnoLIA, Innogenetics). In our experience anti-DNA antibodies do not cross-react with proteins A and D in this blot system. Hence this new blot system avoids the cross-reactivity problems in the assessment of antinuclear antibody specificity for both Sm and U(1)snRNP.

引用

页码：129 / 132

页数：4

共 7 条

[1]

CRAFT J, 1992, RHEUM DIS CLIN N AM, V18, P311

[2] THE 1ST INTERNATIONAL STANDARD FOR ANTIBODIES TO DOUBLE-STRANDED DNA [J].