Detection of Francisella piscicida in Atlantic cod (Gadus morhua L) by the loop-mediated isothermal amplification (LAMP) reaction

被引：11

作者：

Caipang, Christopher M. A. ^{[1
]}

Kulkarni, Amod ^{[1
]}

Brinchmann, Monica F. ^{[1
]}

Korsnes, Kjetil ^{[1
]}

Kiron, Viswanath ^{[1
]}

机构：

[1] Bodo Univ Coll, Fac Biosci & Aquaculture, N-8049 Bodo, Norway

来源：

VETERINARY JOURNAL | 2010年 / 184卷 / 03期

关键词：

Atlantic cod; Gadus morhua; Francisella piscicida; Francisellosis; Loop-mediated isothermal amplification (LAMP); RAPID DETECTION; FISH; BACTERIUM; DISEASE; MORTALITY; PCR;

D O I：

10.1016/j.tvjl.2009.03.027

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The loop-mediated isothermal amplification (LAMP) reaction was evaluated for its speed and sensitivity in detecting the presence of Francisella piscicida, the causative agent of francisellosis in Atlantic cod (Gadus morhua). Four primer sets, consisting of two outer and two inner, were designed from the groEL gene of the pathogen. The LAMP reaction was optimised at 63 degrees C for 1 h using bacterial genomic DNA as the template and the products were visualised under ultra-violet light and analysed by agarose gel electrophoresis. A ladder-like pattern of bands, specific for F. piscicida, was amplified from positive samples. The method was highly specific for the detection of F. piscicida and was 100 times more sensitive than conventional PCR. In addition, the LAMP assay was able to detect the pathogen in kidney and splenic samples of naturally-infected Atlantic cod. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：357 / 361

页数：5

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