Detection of single cells of Cryptococcus neoformans in clinical samples by solid-phase cytometry

被引：10

作者：

Bauters, TGM

Swinne, D

Stove, V

Nelis, HJ

机构：

[1] State Univ Ghent, Lab Pharmaceut Microbiol, Dept Pharmaceut Anal, B-9000 Ghent, Belgium

[2] State Univ Ghent Hosp, Dept Clin Chem Microbiol & Immunol, B-9000 Ghent, Belgium

[3] Inst Trop Med, Dept Clin Sci, B-2000 Antwerp, Belgium

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 04期

关键词：

D O I：

10.1128/JCM.41.4.1736-1737.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml.

引用

页码：1736 / 1737

页数：2

共 6 条

[1]

Bauters TGM, 2001, MED MYCOL, V39, P379, DOI 10.1080/mmy.39.4.379.381

[2] Detection of Cryptococcus neoformans DNA in tissue samples by nested and real-time PCR assays [J].