Escherichia coli transformant expressing the glucose dehydrogenase gene from Bacillus megaterium as a cofactor regenerator in a chiral alcohol production system

被引：50

作者：

Kataoka, M ^{[1
]}

Rohani, LPS

Wada, M

Kita, K

Yanase, H

Urabe, I

Shimizu, S

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 60601, Japan

[2] Tottori Univ, Fac Engn, Dept Biotechnol, Tottori 680, Japan

[3] Osaka Univ, Fac Engn, Dept Biotechnol, Osaka 565, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 01期

关键词：

glucose dehydrogenase; Bacillus megaterium; NADPH regeneration; aldehyde reductase; asymmetric reduction;

D O I：

10.1271/bbb.62.167

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP(+) and glucose, similarly to commercially available GDH.

引用

页码：167 / 169

页数：3

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