Three kinds of binding site for tentoxin on isolated chloroplast coupling factor 1

被引：17

作者：

Mochimaru, M ^{[1
]}

Sakurai, H ^{[1
]}

机构：

[1] Waseda Univ, Sch Educ, Dept Biol, Shinjuku Ku, Tokyo 16950, Japan

来源：

FEBS LETTERS | 1997年 / 419卷 / 01期

关键词：

chloroplast ATP synthase; chloroplast coupling factor 1; F-1-ATPase; energy-transfer inhibitor; tentoxin;

D O I：

10.1016/S0014-5793(97)01421-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tentoxin binding on chloroplast coupling factor 1 (CF1) was studied using a centrifugation column method followed by HPLC analysis. From non-linear regression analysis of the results, the presence of three types of binding site with the following K-d values was deduced: 6.9 X 10(-8) M (first site), 1.4 X 10(-5) M (second site), and 6.3 X 10(-3) M (third site). The binding of one tentoxin inhibits, that of two tentoxins moderately restores, and that of three tentoxins greatly stimulates the ATPase activity of CF1. The forward rate constant of the binding of tentoxin on the first site was 6.3 X 10(3) M-1 s(-1). (C) 1997 Federation of European Biochemical Societies.

引用

页码：23 / 26

页数：4

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