Hypermutation, diversity and dissemination of human intestinal lamina propria plasma cells

被引:73
作者
DunnWalters, DK [1 ]
Boursier, L [1 ]
Spencer, J [1 ]
机构
[1] UMDS,DEPT HISTOPATHOL,LONDON SE1 7EH,ENGLAND
关键词
immunoglobulin gene; lamina propria; plasma cell;
D O I
10.1002/eji.1830271131
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In this work we have microdissected lamina propria plasma cells and used polymerase chain reaction and sequencing to investigate immunoglobulin (Ig) gene rearrangements and mutations in human intestine. In addition, specific primers were designed for individual Ig gene rearrangements to analyze the distribution of related B cell and plasma cell clones at different sites along the bowel. Confirming our earlier work, intestinal IgV(H) genes were highly mutated in plasma cells from older individuals (> 30 years). IgV(H) genes were significantly less mutated in samples taken from patients aged 11-30 years, and there were fewer mutations again in samples from young children (< 11 years). In age-matched specimens the number of mutations was equivalent in the duodenum and colon. Using complementarity-determining region 3 primers to amplify specific Ig gene rearrangements, evidence was also found for the existence of related lamina propria plasma cells along the small bowel and colon, although these were quite scarse. In addition, analysis of the numbers of related clones in a random sampling from discrete areas of lamina propria indicates that the local population is diverse. These results suggest that the highly mutated IgV(H) genes in adult intestinal plasma cells are a consequence of chronic antigen exposure with age. Duodenal plasma cells are as highly mutated as colonic plasma cells, despite the fact that the upper bowel has no indigenous microbial flora (the stimulus for intestinal plasma cells). They also show that the plasma cell population is diverse and can be widely disseminated along the bowel.
引用
收藏
页码:2959 / 2964
页数:6
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