Both B and T lymphocytes may be clonally involved in myelofibrosis with myeloid metaplasia

被引:70
作者
Reeder, TL [1 ]
Bailey, RJ [1 ]
Dewald, GW [1 ]
Tefferi, A [1 ]
机构
[1] Mayo Clin & Mayo Fdn, Div Hematol, Rochester, MN 55905 USA
关键词
D O I
10.1182/blood-2002-07-2341
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
A combination of magnetic cell sorting (MACS) and fluorescent in situ hybridization (FISH) techniques was used to detect clonal cytogenetic markers in different myeloid and lymphoid cell types of the peripheral blood from 4 patients with myelofibrosis with myeloid metaplasia (MMM) that was associated with either a 13q- or a 20q- karyotypic abnormality. Interphase cytogenetics studies demonstrated abnormal clonal FISH signal patterns in neutrophil, myeloid, erythroid, megakaryocyte, and B- and T-cell preparations in 3 of the 4 patients. In one patient, FISH results were within normal limits in T cells and slightly abnormal in B cells. In general, the percentage of abnormal nuclei was variable in both lymphocyte populations but always higher in B lymphocytes compared with T lymphocytes. The current study provides direct evidence for the clonal involvement of both B and T lymphocytes in MMM. A larger study is needed to clarify the relevance of the observed interpatient heterogeneity in clonal constitution.
引用
收藏
页码:1981 / 1983
页数:3
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