Identification and quantification of ovine gammaherpesvirus 2 DNA in fresh and stored tissues of pigs with symptoms of porcine malignant catarrhal fever

被引:41
作者
Albini, S
Zimmermann, W
Neff, F
Ehlers, B
Häni, H
Li, H
Hüssy, D
Engels, M
Ackermann, M
机构
[1] Univ Zurich, Inst Virol, Fac Med Vet, CH-8057 Zurich, Switzerland
[2] Univ Bern, Dept Clin Vet Med, CH-3012 Bern, Switzerland
[3] Univ Bern, Inst Anim Pathol, CH-3012 Bern, Switzerland
[4] Robert Koch Inst, D-1000 Berlin, Germany
[5] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA
关键词
D O I
10.1128/JCM.41.2.900-904.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Cases of porcine malignant catarrhal fever were analyzed by a combination of identification and quantitation of ovine gammaherpesvirus 2 DNA in a variety of paraffin-embedded tissues from diseased pigs, serology, and exclusion of primary porcine gammaherpesviruses. In spite of reduced signal due to fixation and paraffin embedding, ovine gammaherpesvirus 2 DNA in pig brains exceeded the amounts found in sheep brains by orders of magnitude.
引用
收藏
页码:900 / 904
页数:5
相关论文
共 27 条
[1]   PCR DETECTION OF THE SHEEP-ASSOCIATED AGENT OF MALIGNANT CATARRHAL FEVER [J].
BAXTER, SIF ;
POW, I ;
BRIDGEN, A ;
REID, HW .
ARCHIVES OF VIROLOGY, 1993, 132 (1-2) :145-159
[2]  
BRATBERG B, 1980, 10 C INT PIG VET SOC, P353
[3]   DERIVATION OF A DNA CLONE CORRESPONDING TO THE VIRAL AGENT OF SHEEP-ASSOCIATED MALIGNANT CATARRHAL FEVER [J].
BRIDGEN, A ;
REID, HW .
RESEARCH IN VETERINARY SCIENCE, 1991, 50 (01) :38-44
[4]   AN EPIZOOTIC OF MALIGNANT CATARRHAL FEVER IN A LARGE CAPTIVE HERD OF WHITE-TAILED DEER (ODOCOILEUS-VIRGINIANUS) [J].
BROWN, CC ;
BLOSS, LL .
JOURNAL OF WILDLIFE DISEASES, 1992, 28 (02) :301-305
[5]   Detection and multigenic characterization of a novel gammaherpesvirus in goats [J].
Chmielewicz, B ;
Goltz, M ;
Ehlers, B .
VIRUS RESEARCH, 2001, 75 (01) :87-94
[6]   Detection of two novel porcine herpesviruses with high similarity to gammaherpesviruses [J].
Ehlers, B ;
Ulrich, S ;
Goltz, M .
JOURNAL OF GENERAL VIROLOGY, 1999, 80 :971-978
[7]   Detection of new DNA polymerase genes of known and potentially novel herpesviruses by PCR with degenerate and deoxyinosine-substituted primers [J].
Ehlers, B ;
Borchers, K ;
Grund, C ;
Frölich, K ;
Ludwig, H ;
Buhk, HJ .
VIRUS GENES, 1999, 18 (03) :211-220
[8]   Characterization of the DNA polymerase loci of porcine cytomegaloviruses from diverse geographic origins [J].
Goltz, M ;
Widen, F ;
Banks, M ;
Belak, S ;
Ehlers, B .
VIRUS GENES, 2000, 21 (03) :249-255
[9]  
Holmgren N., 1983, Svensk Veterinartidning, V35, P103
[10]   Analysis of the pathogenetic basis for shedding and transmission of ovine gamma herpesvirus 2 [J].
Hüssy, D ;
Janett, F ;
Albini, S ;
Stäuber, N ;
Thun, R ;
Ackermann, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (12) :4700-4704