Microinjection of Cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes

被引：22

作者：

de Wit, T ^{[1
]}

Drabek, D ^{[1
]}

Grosveld, F ^{[1
]}

机构：

[1] Erasmus Univ, Dept Cell Biol, MGC, NL-3000 DR Rotterdam, Netherlands

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 02期

关键词：

D O I：

10.1093/nar/26.2.676

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a strategy for producing single copy transgenic mouse lines using Cre-loxP site specific recombination. The method is based on transient expression of the recombinase after injection of in vitro transcribed mRNA into the cytoplasm of fertilised eggs containing multiple copies of the transgene, The success rate of the recombination event is 100% (15 out of 15).

引用

页码：676 / 678

页数：3

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