The role of oxidized low-density lipoprotein in breaking peripheral Th17/Treg balance in patients with acute coronary syndrome

被引：80

作者：

Li, Qing ^{[1
,2
,3
,4
]}

Wang, Yi ^{[1
,2
,3
]}

Chen, Ke ^{[5
]}

Zhou, Qing ^{[1
,2
,3
]}

Wei, Wei ^{[2
,3
]}

Wang, Yiping ^{[6
]}

Wang, Yuan ^{[1
,2
,3
]}

机构：

[1] Anhui Med Univ, Mol Biol Lab, Hefei 230032, Anhui, Peoples R China

[2] Anhui Med Univ, Inst Clin Pharmacol, Hefei 230032, Anhui, Peoples R China

[3] Anhui Med Univ, Minist Educ & Anhui Prov, Key Lab Gene Resource Utilizat Severe Dis & Antii, Hefei 230032, Anhui, Peoples R China

[4] Anhui Prov Hosp, Med Res Ctr, Cent Lab, Hefei 230001, Anhui, Peoples R China

[5] Anhui Prov Hosp, Dept Cardiovasc Dis, Hefei 230001, Anhui, Peoples R China

[6] Univ Sydney, Western Clin Sch, Ctr Transplantat & Renal Res, Westmead, NSW 2145, Australia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 394卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Oxidized low-density lipoprotein; Acute coronary syndrome; T-helper; 17; Regulatory T cells; REGULATORY T-CELLS; ARTERY-DISEASE; ATHEROSCLEROSIS; IMMUNITY; INNATE;

D O I：

10.1016/j.bbrc.2010.03.090

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Oxidized low-density lipoprotein (ox-LDL) is an instrumental factor in atherogenesis. however, the effects of ox-LDL on the balance of Th17/Treg in acute coronary syndrome [ACS, including unstable angina (UA) and acute myocardial infarction (AMI)] is still unclear CD4'CD25(+). regulatory T (Treg) cells and Till 7 cells, subsets of T-helper cells, play important roles in peripheral immunity and their imbalance leads to the development of tissue inflammation and autoimmune diseases However, few studies have explored the effect of Th17/Treg balance in plaque destabilization and the onset of ACS To explore the shift of Th17/Treg balance in ACS patients and the effect of ox-LDL on the balance, we examined the frequencies of Th17 and Treg cells, key transcription factors and relevant cytokines in patients with AMI. UA, stable angina (SA) and controls We analysed the correlations of serum ox-LDL to Th17/Treg frequency, and the effects of ox-LDL on Th17/Treg cells in vitro Our study demonstrated that ACS patients have shown a significant increase of Th17 frequency. ROR gamma t expression and serum Interleukin 17 (IL-17), and a obvious decline of Treg frequency, Foxp3 expression, suppressive function, and serum IL-10 Serum ox-LDL positively correlated with the frequency of Th17 cells and negatively correlated with the frequency of Treg cells In vitro incubation of peripheral blood mononuclear cells from controls with ox-LDL resulted in a significant reduction of Treg cells and a significant elevation of Th17 cells in a dose- and time-dependant manner Treg and Th17 cells from ACS patients were significantly more susceptible to ox-LDL-mediated alterations Th17/Treg numerical and functional imbalance exists in ACS patients, and ox-LDL has a direct effect on Th17/Treg imbalance which may contribute to the occurrence of ACS (C) 2010 Elsevier Inc All rights reserved

引用

页码：836 / 842

页数：7

共 20 条

[1]

Human serum paraoxonase (PON 1) is inactivated by oxidized low density lipoprotein and preserved by antioxidants [J].