Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes (vol 11, pg 412, 2004)

被引：13

作者：

Harel, M

Aharoni, A

Gaidukov, L

Brumshtein, B

Khersonsky, O

Meged, R

Dvir, H

Ravelli, RBG

McCarthy, A

Toker, L

Silman, I

Sussman, JL

Tawfik, DS

机构：

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2004年 / 11卷 / 12期

关键词：

D O I：

10.1038/nsmb1204-1253a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nat. Struct. Mol. Biol. 11, 412–419 (2004). Some of the enzymatic activity data for paraoxonase (PON1) reported in this article were biased by two factors. First, the gene encoding the H115Q mutant contained an additional H177R mutation introduced during PCR amplification. Although His177 is far from the active site and the H177R mutant by itself is 70% active compared to the wild-type PON1, the combined H115Q H177R mutant is almost completely inactive.

引用

页码：1253 / 1253

页数：1