Vascular smooth muscle cell glycocalyx influences shear stress-mediated contractile response

被引:48
作者
Ainslie, KM
Garanich, JS
Dull, RO
Tarbell, JM
机构
[1] CUNY City Coll, Dept Biomed Engn, New York, NY 10031 USA
[2] Univ Utah, Dept Anesthesiol, Salt Lake City, UT USA
[3] Penn State Univ, Dept Chem Engn, Biomol Transport Dynam Lab, University Pk, PA 16802 USA
[4] Penn State Univ, Dept Bioengn, University Pk, PA 16802 USA
关键词
glycosaminoglycans; myogenic response;
D O I
10.1152/japplphysiol.01006.2003
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
This study addressed the influence of the rate of shear stress application on aortic smooth muscle cell (SMC) contraction and the role of specific glycosaminoglycans in this mechanotransduction. Rat aortic SMCs were exposed to either a step increase in shear stress (0 to 25 dyn/cm(2)) or a ramp increase in shear stress (0 to 25 dyn/cm(2) over 5 min) in a parallel plate flow chamber, and cell contraction was characterized by cell area reduction. SMCs contracted at levels similar to those reported previously and equally in response to both a step and ramp increase in shear stress. When the cells were pretreated with heparinase III or chondroitinase ABC to remove the glycosaminoglycans heparan sulfate and chondroitin sulfate, respectively, from the glycocalyx, the contraction response to increases in shear stress was significantly inhibited. These studies indicate that specific components of the SMC glycocalyx play an important role in the mechanotransduction of shear stress into a contractile response and that the rate of application of shear stress does not affect the SMC contraction.
引用
收藏
页码:242 / 249
页数:8
相关论文
共 52 条
  • [1] ADAMSON RH, 1990, J PHYSIOL-LONDON, V428, P1
  • [2] Shear stress-induced release of PGE(2) and PGI(2) by vascular smooth muscle cells
    Alshihabi, SN
    Chang, YS
    Frangos, JA
    Tarbell, JM
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 224 (03) : 808 - 814
  • [3] Phorbol esters increase MLC phosphorylation and actin remodeling in bovine lung endothelium without increased contraction
    Bogatcheva, NV
    Verin, AD
    Wang, PY
    Birukova, AA
    Birukov, KG
    Mirzopoyazova, T
    Adyshev, DM
    Chiang, ET
    Crow, MT
    Garcia, JGN
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2003, 285 (02) : L415 - L426
  • [4] BOURIN MC, 1990, J BIOL CHEM, V265, P15424
  • [5] The cytoplasmic domain of syndecan-1 is required for cytoskeleton association but not detergent insolubility - Identification of essential cytoplasmic domain residues
    Carey, DJ
    Bendt, KM
    Stahl, RC
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (25) : 15253 - 15260
  • [6] Carey DJ, 1997, BIOCHEM J, V327, P1
  • [7] PROTEOGLYCANS IN ARTERIAL SMOOTH-MUSCLE CELL-CULTURES - AN ULTRASTRUCTURAL HISTOCHEMICAL ANALYSIS
    CHEN, KF
    WIGHT, TN
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1984, 32 (04) : 347 - 357
  • [8] Smooth muscle cells contract in response to fluid flow via a Ca2+-independent signaling mechanism
    Civelek, M
    Ainslie, K
    Garanich, JS
    Tarbell, JM
    [J]. JOURNAL OF APPLIED PHYSIOLOGY, 2002, 93 (06) : 1907 - 1917
  • [9] Strain and strain rate activation of G proteins in human endothelial cells
    Clark, CB
    McKnight, NL
    Frangos, JA
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 299 (02) : 258 - 262
  • [10] MECHANISMS OF ARTERIAL GRAFT FAILURE .2. CHRONIC ENDOTHELIAL AND SMOOTH-MUSCLE CELL-PROLIFERATION IN HEALING POLYTETRAFLUOROETHYLENE PROSTHESES
    CLOWES, AW
    KIRKMAN, TR
    CLOWES, MM
    [J]. JOURNAL OF VASCULAR SURGERY, 1986, 3 (06) : 877 - 884