Kiss and run exocytosis at hippocampal synapses

被引：194

作者：

Stevens, CF

Williams, JH

机构：

[1] Salk Inst Biol Studies, Howard Hughes Med Inst, La Jolla, CA 92037 USA

[2] Salk Inst Biol Studies, Mol Neurobiol Lab, La Jolla, CA 92037 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 23期

关键词：

D O I：

10.1073/pnas.230438697

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have combined electrophysiology and imaging to measure the release of neurotransmitter and fluorescent dye at synapses of cultured hippocampal neurons. These experiments have revealed a "kiss and run" mode of exocytosis in which synaptic vesicles release glutamate normally but do not permit dye to enter or escape from the vesicle. During "kiss and run." the vesicle interior may be exposed very transiently(<6 ms), or a special configuration of the fusion pore may prevent dye exchange. We estimate that about 20% of the vesicles normally use this "kiss and run" pathway, and that the fraction of "kiss and run" events can be increased to over 80% by superfusing the synapses with hypertonic solution.

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页码：12828 / 12833

页数：6

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