Intrathecal administration of a new nitric oxide donor, NOC-18, produces acute thermal hyperalgesia in the rat

被引:35
作者
Inoue, T [1 ]
Mashimo, T [1 ]
Shibuta, S [1 ]
Yoshiya, I [1 ]
机构
[1] Osaka Univ, Sch Med, Dept Anesthesiol, Osaka 565, Japan
关键词
nitric oxide; NO-releasing compounds; nociception; hyperalgesia; cyclic GMP; N-nitro-L-arginine methyl ester; methylene blue; hemoglobin; spinal cord;
D O I
10.1016/S0022-510X(97)00188-3
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
A nitric oxide releasing compound, NOC-18, was injected intrathecally in order to determine the role of NO in spinal nociceptive mechanisms in rats. The nociceptive threshold was evaluated by the radiant heat tail-flick test. The effects of intrathecal injection of N-nitro-L-arginine methyl ester (L-NAME), an NO synthase inhibitor; methylene blue (MB), a soluble guanylate cyclase inhibitor and hemoglobin (Hb), an NO scavenger, on the nociceptive threshold were measured in the presence and absence of 0.1, 1 and 10 mu g of NOC-18. The results were compared with a control group of rats which were injected with the same volume of normal saline. NOC-18 caused a dose-dependent curtailment of the tail-flick latency during the period from 15 to 150 min. L-NAME, MB and Hb all produced prolongation of the tail-flick latency during the same time period. The hyperalgesia induced by this concentration range of NOC-18 was completely blocked by Hb, but was not affected by either L-NAME or MB. These findings indicate that NO plays a direct role in thermal hyperalgesia in the spinal cord, and that an another pathway in addition to the NO-cGMP pathway may be involved. (C) 1997 Elsevier Science B.V.
引用
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页码:1 / 7
页数:7
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