Different role of saturated and unsaturated fatty acids in β-cell apoptosis

被引:113
作者
Eitel, K
Staiger, H
Brendel, MD
Brandhorst, D
Bretzel, RG
Häring, HU
Kellerer, M
机构
[1] Univ Tubingen, D-72076 Tubingen, Germany
[2] Univ Giessen, D-35392 Giessen, Germany
关键词
apoptosis; beta-cells; free fatty acids;
D O I
10.1016/S0006-291X(02)02752-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is believed that free fatty acids contribute to the pathogenesis of type 2 diabetes in humans. We have recently shown that lipoapoptosis of human beta-cells is specifically induced by saturated fatty acids while unsaturated had no effect. In the present study we tested the effect of co-incubation of different saturated and unsaturated free fatty acids on lipoapoptosis in beta-cells. RIN1046-38 cells and isolated human beta-cells were incubated with combinations of saturated fatty acids (palmitate, stearate) and mono- or polyunsaturated fatty acids (palmitoleate, oleate, and linoleate). Cells were incubated for 24-72 h with 1 mM fatty acids. All unsaturated fatty acids tested completely prevented palmitate- or stearate-induced apoptosis of rat and human beta-cells as assessed by flow cytometric cell cycle analysis,and TUNEL assay. This might suggest that apoptosis in vivo is predominantly determined by the content of unsaturated fatty acids in a mixed fatty acid pool. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:853 / 856
页数:4
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