Identification of the TYKY homologous subunit of complex I from Neurospora crassa

被引：9

作者：

Duarte, M

Schulte, U

Videira, A ^{[1
]}

机构：

[1] Univ Porto, Inst Ciencias Biomed Abel Salazar, P-4050 Porto, Portugal

[2] Univ Porto, Unidade Multidisciplinar Invest Biomed, P-4050 Porto, Portugal

[3] Univ Dusseldorf, Inst Biochem, D-4000 Dusseldorf, Germany

[4] Univ Porto, Inst Mol & Cellular Biol, P-4050 Porto, Portugal

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1997年 / 1322卷 / 2-3期

关键词：

complex I subunit; iron-sulfur cluster N-2; mitochondrion; (Neurospora crassa);

D O I：

10.1016/S0005-2728(97)00084-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polypeptide subunit of complex I from Neurospora crassa, homologous to bovine TYKY, was expressed in Escherichia coli, purified and used for the production of rabbit antiserum. The mature mitochondrial protein displays a molecular mass of 21280 Da and results from cleavage of a presequence consisting of the first 34 N-terminal amino acids of the precursor. This protein was found closely associated with the peripheral arm of complex I. (C) 1997 Elsevier Science B.V.

引用

页码：237 / 241

页数：5

共 37 条

[1]

ALVES PC, 1994, J BIOL CHEM, V269, P7777

[2] NADH - UBIQUINONE OXIDOREDUCTASE FROM BOVINE HEART-MITOCHONDRIA - A 4TH NUCLEAR ENCODED SUBUNIT WITH A HOMOLOG ENCODED IN CHLOROPLAST GENOMES [J].