Natural Hidden Autoantibodies to Tissue Transglutaminase Cross-React with Fibrinogen

被引：4

作者：

Zoeller-Utz, Ingrid M. ^{[2
]}

Esslinger, Birgit ^{[2
]}

Schulze-Krebs, Anja ^{[2
]}

Dieterich, Walburga ^{[1
,2
]}

机构：

[1] Univ Erlangen Nurnberg, Dept Med 1, D-91052 Erlangen, Germany

[2] Univ Erlangen Nurnberg, Med Clin 1, D-91054 Erlangen, Germany

来源：

JOURNAL OF CLINICAL IMMUNOLOGY | 2010年 / 30卷 / 02期

关键词：

Celiac disease; cross-reactivity; fibrinogen; hidden autoantibody; tissue transglutaminase; CELIAC-DISEASE AUTOIMMUNITY; HEAT INACTIVATION; BINDING; SERUM; ANTIBODIES; GLUTEN; RISK; IGG; IDENTIFICATION; ROTAVIRUS;

D O I：

10.1007/s10875-009-9347-z

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Patients with celiac disease display autoantibodies against tissue transglutaminase (TG2), and the high sensitivity and specificity of these autoantibodies render them a reliable tool for diagnosis. However, we found that denatured sera from healthy persons also showed reactivity against TG2. To further examine the specificity of this phenomenon, sera of healthy individuals and celiac patients were denatured by heat or pH shift. Denatured sera of all individuals showed autoantibodies against TG2 in ELISA that could be specifically inhibited by TG2, but the biological role of these autoantibodies remains unknown. The alpha fibrinogen precursor could be isolated as serum protein that reacts with TG2 antibodies and treated sera reacted with fibrinogen in Western blotting. Cross-reactivity of TG2 antibodies with fibrinogen and vice versa was observed. We hypothesise that denaturation of sera reveals hidden autoantibodies against TG2, which might be normally masked by fibrinogen.

引用

页码：204 / 212

页数：9

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