Isolation and characterization of novel marine-derived actinomycete taxa rich in bioactive metabolites

被引:156
作者
Magarvey, NA
Keller, JM
Bernan, V
Dworkin, M
Sherman, DH
机构
[1] Univ Michigan, Inst Life Sci, Dept Med Chem, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Inst Life Sci, Dept Chem, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Inst Life Sci, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA
[4] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA
[5] Univ Minnesota, Inst Biotechnol, Minneapolis, MN 55455 USA
[6] Wyeth Ayerst Res, Dept Nat Prod Res, Chem Sci Sect, Pearl River, NY USA
关键词
D O I
10.1128/AEM.70.12.7520-7529.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A unique selective enrichment procedure has resulted in the isolation and identification of two new genera of marine-derived actinobacteria. Approximately 90% of the microorganisms cultured by using the presented method were from the prospective new genera, a result indicative of its high selectivity. In this study, 102 actinomycetes were isolated from subtidal marine sediments collected from the Bismarck Sea and the Solomon Sea off the coast of Papua New Guinea. A combination of physiological parameters, chemotaxonomic characteristics, distinguishing 16S rRNA gene sequences, and phylogenetic analysis based on 16S rRNA genes provided strong evidence for the two new genera (represented by strains of the PNG1 clade and strain UMM518) within the family Micromonosporaceae. Biological activity testing of fermentation products from the new marine-derived actinomycetes revealed that several had activities against multidrug-resistant grant-positive pathogens, malignant cells, and vaccinia virus replication.
引用
收藏
页码:7520 / 7529
页数:10
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