Characteristics of a low affinity passive Ca2+ influx component in rat parotid gland basolateral plasma membrane vesicles

被引:6
作者
Chauthaiwale, JV [1 ]
Lockwich, TP [1 ]
Ambudkar, IS [1 ]
机构
[1] NIDR, NIH, Secretory Physiol Sect, Gene Therapy & Therapeut Branch, Bethesda, MD 20892 USA
关键词
Ca2+ influx; parotid gland; BLMV; kinetics; divalent cations;
D O I
10.1007/s002329900351
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously reported the presence of two Ca2+ influx components with relatively high (K-Ca = 152 +/- 79 mu M) and low (K-Ca = 2.4 +/- 0.9 mM) affinities for Ca2+ in internal Ca2+ pool-depleted rat parotid acinar cells [Chauthaiwale et al. (1996) Pfluegers Arch. 432:105-111]. We have also reported the presence of a high affinity Ca2+ influx component with K-Ca = 279 +/- 43 mu M in rat parotid gland basolateral plasma membrane vesicles (BLMV). [Lockwich, Kim & Ambudkar (1994) J. Membrane Biol. 141:289-296]. The present studies show that a low affinity Ca2+ influx component is also present in BLMV with K-Ca = 2.3 +/- 0.41 mM (V-max = 16.36 +/- 4.11 nmoles of Ca2+/mg protein/min). Our data demonstrate that this low affinity component is similar to the low affinity Ca2+ influx component that is activated by internal Ca2+ store depletion in dispersed parotid gland acini by the following criteria: (i) similar K-Ca, for calcium flux, (ii) similar IC50 for inhibition by Ni2+ and Zn2+; (iii) increase in K-Ca at high external K+, (iv) similar effects of external pH. The high affinity Ca2+ influx in cells is different from the low affinity Ca2+ influx component cells in its sensitivity to pH, KCl, Zn2+ and Ni2+. The low and high affinity Ca2+ influx components in BLMV can also be distinguished from each other based on the effects of Zn2+, Ni2+, KCl, and dicyclohexylcarbodiimide. In aggregate, these data demonstrate the presence of a low affinity passive Ca2+ influx pathway in BLMV which displays characteristics similar to the low affinity Ca2+ influx component detected in parotid acinar cells following internal Ca2+ store depletion.
引用
收藏
页码:139 / 145
页数:7
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