Dynamin is membrane-active: Lipid insertion is induced by phosphoinositides and phosphatidic acid

被引:75
作者
Burger, KNJ
Demel, RA
Schmid, SL
de Kruijff, B
机构
[1] Univ Utrecht, Ctr Biomembranes & Lipid Enzymol, Dept Biochem & Membranes, Inst Biomembranes, NL-3584 CH Utrecht, Netherlands
[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA
关键词
D O I
10.1021/bi000971r
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Dynamin is a large GTPase involved in the regulation of membrane constriction and fission during receptor-mediated endocytosis. Dynamin contains a pleckstrin-homology domain which is essential for endocytosis and which binds to anionic phospholipids. Here, we show for the first time that dynamin is a membrane-active molecule capable of penetrating into the acyl chain region of membrane lipids. Lipid penetration is strongly stimulated by phosphatidic acid (PA), phosphatidylinositol 4-phosphate, and phosphatidylinositol 4,5-bisphosphate. Though binding is more efficient in the presence of the phosphoinositides, a much larger part of the dynamin molecule penetrates into PA-containing mixed lipid systems. Thus, local lipid metabolism will dramatically influence dynamin-lipid interactions, and dynamin-lipid interactions are likely to play an important role in dynamin-dependent endocytosis. Our data suggest that dynamin is directly involved in membrane destabilization, a prerequisite to membrane fission.
引用
收藏
页码:12485 / 12493
页数:9
相关论文
共 54 条
[1]  
Achiriloaie M, 1999, MOL CELL BIOL, V19, P1410
[2]   Synergistic activation of dynamin GTPase by Grb2 and phosphoinositides [J].
Barylko, B ;
Binns, D ;
Lin, KM ;
Atkinson, MAL ;
Jameson, DM ;
Yin, HL ;
Albanesi, JP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (06) :3791-3797
[3]   PENETRATION OF THE SIGNAL SEQUENCE OF ESCHERICHIA-COLI PHOE PROTEIN INTO PHOSPHOLIPID MODEL MEMBRANES LEADS TO LIPID-SPECIFIC CHANGES IN SIGNAL PEPTIDE STRUCTURE AND ALTERATIONS OF LIPID ORGANIZATION [J].
BATENBURG, AM ;
DEMEL, RA ;
VERKLEIJ, AJ ;
DEKRUIJFF, B .
BIOCHEMISTRY, 1988, 27 (15) :5678-5685
[4]   The PH superfold: a structural scaffold for multiple functions [J].
Blomberg, N ;
Baraldi, E ;
Nilges, M ;
Saraste, M .
TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (11) :441-445
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]   Functions of lipid rafts in biological membranes [J].
Brown, DA ;
London, E .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1998, 14 :111-136
[7]   Greasing membrane fusion and fission machineries [J].
Burger, KNJ .
TRAFFIC, 2000, 1 (08) :605-613
[8]   INTERACTION OF INFLUENZA-VIRUS HEMAGGLUTININ WITH A LIPID MONOLAYER - A COMPARISON OF THE SURFACE-ACTIVITIES OF INTACT VIRIONS, ISOLATED HEMAGGLUTININS, AND A SYNTHETIC FUSION PEPTIDE [J].
BURGER, KNJ ;
WHARTON, SA ;
DEMEL, RA ;
VERKLEIJ, AJ .
BIOCHEMISTRY, 1991, 30 (46) :11173-11180
[9]  
Burger KNJ, 1997, CURR TOP MEMBR, V44, P403
[10]   INDUCTION OF MUTANT DYNAMIN SPECIFICALLY BLOCKS ENDOCYTIC COATED VESICLE FORMATION [J].
DAMKE, H ;
BABA, T ;
WARNOCK, DE ;
SCHMID, SL .
JOURNAL OF CELL BIOLOGY, 1994, 127 (04) :915-934